In Vivo Application of Labeled Cells
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Principal Investigator for Radiology: Monique R Bernsen, PhD
Our experience and knowledge regarding cell labeling and cell imaging is used in translational research regarding stem cell therapy in collaboration with other departments in the Erasmus MC. Two main in vivo applications for stem cell therapy are being studied.
Stem cell therapy for myocardial infarction
In collaboration with the Department of Cardiology we are implementing cell imaging using high resolution MRI to study clinically relevant principles of cardiac stem cell therapy in small laboratory animals on a clinical scanner. Using the standard clinical imaging gradients, pulse sequences and ECGs, in combination with sensitive small radius surface coils, we are able to image all relevant pulse sequences in rats as well as mice (Figure 3). While visualization of labeled cells in tissue from body parts not affected by uncontrolled motion such as breathing and heartbeat is quite feasible with relevant sensitivity and resolution, the sensitivity is expected to be severely limited in cardiac tissue, due to motion artifacts caused by the beating heart. We are currently able to detect 1000 iron-oxide labeled cells and have also imaged cells labeled with a Gd-based contrast agent, resulting in positive contrast. Further efforts will be focused on increasing the detection sensitivity of labeled cells in the myocardium of living animals and looking at therapeutic potential of various cell types.
Figure 3.
Stem cell therapy for musculoskeletal disorders
In collaboration with the Department of Orthopedics we are studying the use of cell imaging by high resolution MRI for cell-based therapy in musculoskeletal disorders. We have assessed the feasibility of labeling mesenchymal stem cells and chondrocytes with iron-oxide nanoparticles. We have already established that both cell types can be labeled with iron-oxide particles and are able to retain the iron oxide particles for several weeks in vitro and in vivo. In addition, we have also demonstrated that MSC can be efficiently labeled with iron-oxide particles without compromising their multi-lineage potential (Figure 2 and Piotr figure 7). Further research efforts will be focused on developing imaging protocols for the detection of labeled cells in affected joints and assessment of therapeutic potential by in vivo imaging.
Funding: Erasmus MC Translational Research Seed Grant 2004-2006: "MRI tracking of transferred cells in vivo".
Participating researchers Dept. of Radiology: Sandra van Tiel , Linda van der Graaf, Robert-Jan van Geuns, Gavin Houston, Gabriel Krestin, Piotr Wielopolski.
Collaborations:
|
Country |
City (State) |
Institute |
Department |
Collaborator |
|
Germany |
Munich |
GE Medical Systems [ASL Europe] |
Timo Schirmer, PhD |
|
|
the Netherlands |
‘s Hertogenbosch |
GE Medical Systems [ASL Europe] |
Gavin C Houston, PhD |
|
|
the Netherlands |
Delft |
Flick Engineering Solutions |
Herman Flick, MEng |
|
|
the Netherlands |
Delft |
University of Technology |
Reactor Instituut |
Gerben A Koning, PhD |
|
the Netherlands |
Eindhoven |
Technical University |
Biomedical Engineering |
Klaas Nicolay, PhD |
|
the Netherlands |
Eindhoven |
Technical University |
Biomedical Engineering |
Gustav J. Strijkers, PhD |
|
the Netherlands |
Enschede |
University of Twente |
Biomedical Optics |
Kiran Kumar Thumma |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Cardiology |
Heleen MM van Beusekom, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Cardiology |
Ewout Jan van den Bos, MD, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Cardiology |
Dirk J Duncker, MD, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Cardiology |
Wim J van der Giessen, MD, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Hepatogastroenterology |
Ernst J Kuipers, MD, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Neonatology |
Ingrid B Renes, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Nuclear Medicine |
Marion de Jong, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Orthopedics |
Eric J Farrell, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Orthopedics |
Gerjo JVM van Osch, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Orthopedics |
Harrie H Weinans, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Plastic & Reconstructive Surgery |
Ronald I Siphanto, MD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Surgical Oncology |
AMM (Lex) Eggermont, MD, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Surgical Oncology |
Timo LM ten Hagen, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Surgical Oncology |
Gerben A Koning, PhD |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Virology |
Fiona Read |
|
the Netherlands |
Rotterdam |
Erasmus MC |
Virology |
Thijs Kuiken, DVM, PhD, DACVP |
|
USA |
Milwaukee (WI) |
GE Medical Systems [ASL Central] |
Jason A Polzin, PhD |
|
|
USA |
San Francisco (CA) |
University of California |
Radiology |
Robert C Brasch, MD |