Sleutels, Frank

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Frank Sleutels started his scientific career receiving his Masters diploma in Molecular Biology at Nijmegen University. He continued within the department of Molecular Genetics of the Netherlands Cancer Institute were he received his PhD (2001) studying the mechanism of genomic imprinting. Important breakthrough from his thesis was the discovery that the imprinting mechanism exploits non-coding RNAs to silence imprinted genes. This key result not only enforced the common features between imprinting and the X-chromosome inactivation mechanism but also stressed the significance of RNAs as gene regulators. During that time, a key regulator of chromatin structure at imprinted loci was discovered, called CTCF. So he moved on to work on chromatin structure and epigenetics controlled by CTCF at the department of Cell Biology at the Erasmus MC supported by an NWO-Veni grant. His lab studies the mechanism of this zinc-fingers protein family in epigenetic gene regulation and chromatin dynamics. We make use of conditional mouse models, genetically engineering of stem cells, live-cell fluorescence imaging, and advanced chromatin assays.

Address  Erasmus MC - Dept of Cell Biology 
room Ee 10-30
PO Box 2040
3000 CA  Rotterdam
The Netherlands 
Phone +31-10-7043166
Emailf.sleutels - at - erasmusmc.nl



Van de Nobelen S, Rosa-Garrido M, Leers J, Heath H, Soochit W, Joosen L, Jonkers I, Demmers J, van der Reijden M, Torrano V, Grosveld F, Delgado MD, Renkawitz R, Galjart N, Sleutels F (2010)
CTCF regulates the local epigenetic state of ribosomal DNA repeats
Epigenetics Chromatin 3:19

Ribeiro de Almeida C, Heath H, Krpic S, Dingjan GM, van Hamburg JP, Bergen I, van de Nobelen S, Sleutels F, Grosveld F, Galjart N, Hendriks RW (2009)
Critical role for the transcription regulator CCCTC-binding factor in the control of Th2 cytokine expression
J Immunol 182:999-1010

Heath H, Ribeiro de Almeida C, Sleutels F, Dingjan G, van de Nobelen S, Jonkers I, Ling KW, Gribnau J, Renkawitz R, Grosveld F, Hendriks RW, Galjart N (2008)
CTCF regulates cell cycle progression of alphabeta T cells in the thymus.
EMBO J 27:2839-2850

Sleutels F, Tjon G, Ludwig T, Barlow DP. (2003)
Imprinted silencing of Slc22a2 and Slc22a3 does not need transcriptional overlap between Igf2r and Air
EMBO J 22:3696-3704

Sleutels F, Zwart R, Barlow DP. (2002)
The non-coding Air RNA is required for silencing autosomal imprinted genes
Nature 415:810-813

Zwart R, Sleutels F, Wutz A, Schinkel AH, Barlow DP. (2001)
Bidirectional action of the Igf2r imprint control element on upstream and downstream imprinted genes
Genes Dev 15:2361-2366

Sleutels F, Barlow DP. (2001)
Investigation of elements sufficient to imprint the mouse Air promoter
Mol Cell Biol 21:5008-5017