4Pi
Leica 4Pi room Be 336c (4Pi336)
Lasers: Argon (458, 488, 514), Diode (568), HeNe (633), Mai tai tunable multi-photon laser (710-950nm)
Lenses: 2x HCX PL APO 100x glycerol N.A. 1.35 0.22/0.22
Detectors: 4x Spectral PMT's, 2x Avalanche diodes
Main Usage: Super resolution 3D scans
Contact person: Gert van Cappellen
4Pi Principle
The excitation spot is split in 1 main lobe and 2 side lobes (Multi-photon excitation). This smaller main lobes gives the very high resolution.
Reality, 100 nm bead XZ-image
The raw data of the 4Pi microscope will contain the side lobe information (XZ image beta-tubulin).
After deconvolution (correction of side lobes) a high resolution image remains
To give a better impression look at the volume rendered hela cell with beta-tubulin staining.
Gert van Cappellen, beta-tubulin slides made by Jeffrey van Haren