Nuclear Dynamics of PCNA in DNA
Replication and Repair
Jeroen Essers, Arjan F. Theil, Céline Baldeyron, Wiggert A. van Cappellen, Adriaan B. Houtsmuller, Roland Kanaar, Wim Vermeulen
MCB 2005/2006
| Video: Filmpje over bewegende cellen. Download video nuclmov1 | Time lapse imaging of four cells expressing GFP-hPCNA. Cells were imaged every 10 minutes during a 5-hour period. Shown are the images taken every 60 minutes. All four cells will go through cell division and follow the characteristic PCNA focal pattern. Small dots are characteristic for early S phase, staining at the periphery of the nucleus marks the mid S phase and big blobs mark the late S phase. The G2 cell divided first and depending on stage in S-phase the othercells followed. |
| Video: Filmpje 2 van Nuclear Dynamics of PCNA in DNA Download video nuclmov2 | Time-lapse imaging of GFP-hPCNA in cells treated with local UV damage shows transient accumulation of GFP-hPCNA (indicated by the circles) at sites of local UV damage in different stages of the cell cycle. Based on the PCNA replication pattern the upper cell was at the transition from G1 to early S-phase and the lower cell in the early S-phase. In the upper cell, this local GFPhPCNA accumulation disappeared for a short period during early S phase but reappeared at the transition from the early S to mid S phase. In the lower cell the local GFP-hPCNA accumulation disappeared in the G2 and the subsequent G1-phase in the daughter cells. Note the decrease in fluorescence signal around mitosis as a result of cells rounding up and detaching from the coverslips. |