Gallery
Gallery of some confocal images and movies
 | Multi-time multi location experiment. CHO H2b-GFP transfected cells. 12 different locations. Image made every 10 minutes. 6 optical planes. Argon laser, ouput power 50% (6.1A). AOTF 0.5%. Total time 14h, 2.5 GB of data. Gert van Cappellen AVI movie 3 MB 85 frames |
 | Demonstration of the flexibility of the time lapse macro. At one location 2 different scans in time. Low zoom scan every 15 min. Part in the red square zoomed in and scanned every 3 min. Original scans 512x512, 4 planes 10/50 time points, max. projection. For demonstration purpose the laser power was high to bleach the cells in the selected area. In the zoomed in area you see a dividing cell. The cells were kindly provided by Jeroen Essers and were transfected by Evert-Jan Uringa. AVI movie 1.4 Mb 512x256 50 frames |
 | PCNA-GFP CHO9 cells Jeroen Essers 30 images, 5h, every 10 min 512x512x6 confocal planes of 1.5 µm AVI movie 428 Kb 256x256 30 frames |
 | COS cell, CLIP170 (red) Niels Galjart 1024x1024, 2 channels, 8bit |
 | The difference between 2D and 3D. Fixed testis slide (10 µm) 3 years old. The propidium iodide (PI) counter stain is still clearly visible. Willy Baarends, Jan Bijman & Gert van Cappellen AVI movie 814 Kb 258x200 8bit 16 frames |
 | Cardiomyocytes (cultured shortly). Fixed glass cultured cells. Actin fillaments stained with R123phalloidin (Red) and ß1 integrine FITC (green). Bob Scholte, Nico-Dirk van Loo BW AVI movie 770 Kb 256x256 8bit 12 frames |
 | Tile scan of a blood vessel of a GFP mouse. 10x8 tile (4080x2560 pixels, 450 Kb). Caroline Cheng & Rob Krams |
 | cell culture: primary cultured hippocampal neurons, 22 days in vitro. construct: dsred with delayed shift in fluorescence from green-red (Science 290:1585-8, 2000) cells were transfected 24 hrs prior to imaging the image is a magnification of a neurite, recorded for 15 min, two frames/min. Pim French AVI movie 860 Kb 512x218 31 frames |