target menu
 

Guido Jenster

Principal Investigator

Guido Jenster, Ph.D.

Professor of Experimental Urological Oncology
Josephine Nefkens Institute, Room Be362a
Erasmus MC
P.O. box 2040,
3000 CA Rotterdam,
The Netherlands
phone: +31-10-704-3672
fax: +31-10-704-4661
e-mail:g.jenster@erasmusmc.nl



Research interests

Support and involvement in consortium projects

Curriculum Vitae

Staff

Dissertations

Alumni

White papers and Presentations

Publications


Research Interest


The research program of my laboratory is focused on:
1) Characterization of novel markers for prostate cancer (PCa)
2) Identification of genes and pathways responsible for progression of PCa
3) Development of new tools and databases







1) Characterization of novel markers for prostate cancer (PCa)

We identified that exosomes (small vesicles) are released by cancer cells and contain cytoplasmic proteins and RNAs derived from the cells that produced them. These exosomes are therefore considered 'biomarker treasure chests' since their molecular characterization can tell the presence and status of the cancer cells. We have developed an ELISA that can measure these exosomes and are developing nanobodies against PCa-derived exosomes for their specific capture and detection in body fluids.

Using microarray and RNAseq datasets, we identified hundreds of novel RNAs that are only expressed in PCa cells. These transcripts referred to as EPCATs (EMC PCa-associated transcripts) are long noncoding RNAs and perfect diagnostic markers. We are now developing assays to detect EPCATs in urine (exosomes) and in biopsies (by in situ hybridization) to test their diagnostic and prognostic value in a clinical setting.

Besides long noncoding RNAs, we also discovered new small RNAs that are derived from tRNAs and snoRNAs. Some of these RNA fragments have diagnostic and prognostic value and are under investigation.

2) Identification of genes and pathways responsible for progression of PCa

From DNAseq (whole genome and exome seq) and RNAseq, we identified many novel fusion genes and genes mutated in PCa. These genes are being analyzed for their function as potential new oncogenes and tumor suppressor genes in PCa. Besides increasing our understanding of cancer progression, these genes might be targets for therapy.


One pathway we have worked on for years is the androgen receptor cascade. The mechanisms by which PCa becomes castration independent remains an important focus of our group.

3) Development of new tools and databases

An essential layer in our research is the constant development and improvement of technologies, model systems, databases and bioinformatics tools.

In collaboration with the department of Bioinformatics, new software to process DNAseq and RNAseq data is being developed. Various tools to identify novel fusion genes, correct for polymorphisms and coexpression algorithms are under construction. In addition, effort is put into the storage, standard NGS data processing and integration of data.

In order to reproduce the progression of PCa in patients, we constantly develop new model systems including tissue slices, patient-derived xenografts, 2D and 3D cell culture systems and therapy-resistant cell lines.

To further support our ongoing research, we expand and create new databases and biobanks. Extensive efforts are ongoing to sequence fresh frozen and FFPE PCa samples to develop a large dataset for discovery and validation of genes of interest. Biobanks for urine, serum, fresh tissue are expanded to supply the group and collaborators with the materials to move lab discoveries to a pre-clinical validation level.

New techniques and technologies are kept up to date and we invested in the optimization of in situ hybridization, EU-ELISA, nanobody phage display libraries and in vivo imaging technologies.


Support and involvement in consortium projects


  • PRIMA: Prostate cancer integral management approach; EU-FP6 (2003-2008)
  • Nuclear receptors in targeted cancer therapy: improved methods for candidate selection. Top Institute Pharma (2007-2011)
  • CANCURE: Marie Curie Initial Training Network (2006-2010)
  • ProspeR: Prostate cancer: profiling and evaluation of ncRNA; EU-FP7 (2008-2011)
  • PRO-NEST: Prostate Research Organizations-Network of Early Stage Training. Marie Curie Initial Training Network (2009-2013)
  • PCMM: Prostate Cancer Molecular Medicine. Center for Translational Molecular Medicine (2009-2015)
  • TraIT: Translational IT. Center for Translational Molecular Medicine (2011-2015)
  • Movember GAP1 Exosomes (2012-2014)
  • PREDECT: New Models for Preclinical Evaluation of Drug Efficacy in Common Solid Tumors. EU-IMI-EFPIA (2011-2016)
  • NGS-ProToCol: Next Generation Sequencing from Prostate to Colorectal Cancer. -Center for Translational Molecular Medicine (2014-2015)
  • Erasmus MC CCBC: Erasmus MC Cancer Computational Biology Center. Daniel den Hoed Foundation (2014-2016)
  • Cancer-ID: STW-Perspectief : New Technology for monitoring Cancer therapy through extracellular vesicle Identity (2015-2018)
  • CTSF: Erasmus MC Cancer Therapy Screening Facility. Daniel den Hoed Foundation (2015-2017)
  • IMMPROVE : KWF-Alpe d’HuZes: Innovative measurements and markers for prostate cancer diagnosis and prognosis using extracellular vesicles (2016-2020)
  • EffiBody : Eurostars: Development of a new platform technology for efficient, fast and cost-effective antibody generation (2016-2018)

Curriculum Vitae


• 1983-1989: Agricultural University Wageningen, The Netherlands.

• 1990-1994: PhD degree: Department of Endocrinology & Reproduction, Erasmus University Rotterdam, Rotterdam, The Netherlands

Jenster proefschrift

Dissertation: Functional Domains of the Human Androgen Receptor.

Promotor: Prof.dr. J.A. Grootegoed, co-promotors: Dr. A.O. Brinkmann and Prof.dr. ir. J. Trapman.

• 1994-1997: Post-doctoral Associate: Department of Cell Biology, Baylor College of Medicine, Houston, Texas, USA. Laboratory of Dr. Ming-Jer Tsai and Dr. Sophia Tsai under supervision of Dr. Bert W. O'Malley:

• 1997-1999: Assistant Geneticist and Assistant Professor of Urology

Department of Urology and Department of Genetics, M.D. Anderson Cancer Center, University of Texas, Urologic Oncology Research Laboratory, Houston, Texas, USA.

• 1999-2006: Assistant Professor of Urology, Erasmus MC, Rotterdam, The Netherlands

• 2006-2012: Associate Professor of Urology, Erasmus MC, Rotterdam, The Netherlands

• 2012-current: Professor of Experimental Urological Oncology, Erasmus MC, Rotterdam, The Netherlands



Staff


Diana Brusik-van Houten

Secretary

Harmen van de Werken

Managing Director CCBC

Job van Riet

Bioinformatician CCBC

Elena Martens

Senior postdoc

Rute Marques

Senior postdoc

Hanneke van Zoggel

Postdoc

Wilma Teubel

Research Technician, Lab Manager

Natasja Dits

Research Technician

Mirella van den Berg

Research Technician

Joke Veldhoven-Zweistra

Research Technician

Lisanne Mout

PhD student

Lisanne van Dessel

PhD student

Youri Hoogstrate

PhD student bioinformatics

Saskia Hiltemann

PhD student bioinformatics

Mathijs Scholtes

MSc student


Dissertations


2935_Marques_Rute_Barbosa

Rute Marques
The Role of the Androgen Receptor Pathway in Prostate Cancer Progression (2009)

2869_Heul-Nieuwenhuijsen

Leonie van der Heul-Nieuwenhuijsen
Cancer related gene expression in the human prostate zones (2009)

2993_Wijngaart

Dennis van de Wijngaart
Peptides and proteins interacting with the androgen receptor (2009)

2657_Jelier

Rob Jelier
Text Mining applied to Molecular Biology (2008)

2604_Dekker

Lennard Dekker
Proteomics of body fluids (2007)

Delila Gasi Tanderfelt

Delila Gasi Tanderfelt

Expression and Function of ETS genes in Prostate Cancer (2013)

Flip H. Jansen

Flip H. Jansen
Discovery and Validation of Prostate Cancer Biomarkers (2013)

 

Characterization of Novel Genetic Alterations in Prostate Cancer (2015) 

Ines Teles Alves
Characterization of Novel Genetic Alterations in Prostate Cancer (2015)

Taxanes and Novel Androgen Receptor Targeted Agents in the Management .. 

Robert J. van Soest
Taxanes and Novel Androgen Receptor Targeted Agents in the Management of Metastatic Castration-Resistant Prostate Cancer (2015

 

Discovery and Validation of Prognostic Tissue Markers in Clinical Prostate Cancer 

A. Marije Hoogland
Discovery and Validation of Prognostic Tissue Markers in Clinical Prostate Cancer (2015)


Alumni


2001-2008 Dennis van de Wijngaart (PhD student)
2002-2005 Antoine Veldhoven (Bioinformatician)
2002 Nazmoen Mahmood (BSc student)
2002-2005 Peter Hendriksen (Postdoc)
2002-2006 Rute Marques (PhD student)
2002-2007 Leonie van der Heul-Nieuwenhuijsen (PhD student)
2003 Thao van Nguyen (MSc student)
2004-2006 Gert Jan van der Bemd (Postdoc)
2004-2008 Don de Lange (BSc bioinformatics student and Bioinformatician)
2005 Sheyde Ojaghi (BSc bioinformatics student)
2005 Désirée Peters (BSc bioinformatics student)
2005-2008 Flip Jansen (PhD student)
2006 Armina Malchasian (Medical student)
2006 William van den Berge (BSc student)
2006-2008 Angelique van Rijswijk (Research Assistant)
2007 Ruta Keflom (BSc student)
2007 Farshid Arasteh Samani (BSc student bioinformatics)
2007 Mirella van den Berg (BSc student)
2007-2008 Matthijs Moll (MSc student)
2008 Lilly David (BSc student bioinformatics)
2008 Anouk Heine (BSc student bioinformatics))
2008 Tessa Dorival (BSc student bioinformatics)
2008 Barry van der Mast (BSc student bioinformatics)
2008-2009 Christos Chelonas (MSc student)
2009 Eurydice Martine (BSc student)
2009 Zhian Mahmoud (BSc student)
2009-2010 Bas Pigmans (BSc student bioinformatics)
2006-2009 Rajesh Sookhlall (Research Assistant)
2009-2010 Sanaz Yahyanejad (MSc student)
2010 Stefan Roobol (MSc student)
2010-2011 Anton Kalsbeek (MSc student)
2010-2011 David Cano Rodriguez (MSc student)
2010-2011 Hatice Acar (BSc student)
2010-2011 Nilab Naimi (BSc student)
2011-2012 Youri Hoogstrate (Bioinformatician)
2011-2012 Martijn van der Schoor (BSc student bioinformatics)
2011-2012 Michael Olvedy (MSc)
2011-2012 Fedde Schaeffer (BSc student bioinformatics)
2012 Ricardo Vos (BSc student)
2011-2012 Jos van Nijnatten (BSc student)
2012-2013 Thomas Hartjes (MSc student)
2012-2013 Lizzy van den Berg (Assistant)
2012-2013 Nikolas Strepis (MSc student)
2008-2011 Diederick Duijvesz (PhD student)
2011-2014 Yin Versluis (PhD student)
2012-2013 Chris van der Lans (BSc student bioinformatics)
2013 Maartje van 't Hof (MSc Medical student)
2013 Vincent Roodzant (B AS student)
2013-2014 Christa van der Fels (MSc Medical student)
2013-2014 Piotr Maciej Waranecki (B AS student and Research Assistant)
2013-2014 Wubalem Desta Seifu ((MSc student)
2009-2014 Inês Teles Alves (PhD student)
2010-2014 Giovanny Alejandro Rodriguez Blanco (PhD student)
2014 Charlotte Stokkermans (BSc student)
2012-2014 Robert van Soest (PhD student)
2012-2014 Suzana Vidic (Postdoc)
2014-2015 Wendy Plugge (MSc student)
2012-2015 René Böttcher (PhD student bioinformatics)
2014-2015 Matthijs Bekkers (MSc student)
2014-2015 Mónica Vara Pérez (MSc student)
2014-2015 David Nieuwenhuijse(MSc student and bioinformatician)
2015-2016 Petra van Duijn (Postdoc)
2012-2016 Matthijs Moll (PhD student)



White papers and Presentations



Publications


For a list of publications, click here .