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Sixma, Titia

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Titia Sixma studied biochemistry at Utrecht University, The Netherlands. She obtained her PhD (1992) from Groningen University in the group of Wim Hol, studying the crystal structure of Escherichia coli heat-labile enterotoxin (LT), a close relative of cholera toxin. This bacterial protein toxin resembles cholera toxin and its structure determination opened possibilities for drug design against cholera and traveller’s disease.

After a short postdoctoral period in the group of Paul Sigler (Yale University) she returned as group leader to the Netherlands Cancer Institute (since 1994). In 2001 she obtained tenure at the NKI and in 2004 she became part-time bijzonder professor at Erasmus MC.

The Sixma group studies ubiquitin conjugation and DNA repair using a combination of structural methods and biochemistry in order to understand basic cellular processes.

Sixma group website


Key publications

Hibbert RG, Huang A, Boelens R, Sixma TK (2011)
E3 ligase Rad18 promotes monoubiquitination rather than ubiquitin chain formation by E2 enzyme Rad6
Proc Natl Acad Sci USA 108:5590-5595

Luna-Vargas MP, Faesen AC, van Dijk WJ, Rape M, Fish A, Sixma TK (2011)
Ubiquitin-specific protease 4 is inhibited by its ubiquitin-like domain
EMBO Rep 12:365-372

Lebbink JH, Fish A, Reumer A, Natrajan G, Winterwerp HH, Sixma TK (2010)
Magnesium coordination controls the molecular switch function of DNA mismatch repair protein MutS
J Biol Chem 285:13131-13141

Knipscheer P, Flotho A, Klug H, Olsen JV, van Dijk WJ, Fish A, Johnson ES, Mann M, Sixma TK, Pichler A (2008)
Ubc9 sumoylation regulates SUMO target discrimination
Mol Cell 31:371-382

Knipscheer P, van Dijk WJ, Olsen JV, Mann M, Sixma TK (2007)
Noncovalent interaction between Ubc9 and SUMO promotes SUMO chain formation
EMBO J 26:2797-2807

Ulens C, Hogg RC, Celie PH, Bertrand D, Tsetlin V, Smit AB, Sixma TK (2006)
Structural determinants of selective alpha-conotoxin binding to a nicotinic acetylcholine receptor homolog AChBP
Proc Natl Acad Sci USA 103:3615-3620

Lamers MH, Perrakis A, Enzlin JH, Winterwerp HH, de Wind N, Sixma TK (2000)
The crystal structure of DNA mismatch repair protein MutS binding to a G x T mismatch
Nature 407:711-717

Brejc K, van Dijk WJ, Klaassen RV, Schuurmans M, van Der Oost J, Smit AB, Sixma TK (2001)
Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors
Nature 411:269-276