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Gallery timelapse

Gallery timelapse

 

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Download video H2bhela19loc8align.mp4

 Dividing H2B-GFP hela cells imaged with a confocal microscope using a transmission and fluorescence channel. Exitation 488 nm and emission BP 500-550 nm.
 

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Download video Gfp-clip170 Stable Line

 Plus end binding protein Clip170-GFP in 3T3 cells. Confocal microscope excitation 488 nm, emission BP500-550 nm. Time lapse of 49 time points, every 2 seconds, total 98 seconds. (Tatiana Stepanova, group Niels Galjart, Gert van Cappellen)
 

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Download video Birthhaemstemcellcomplaorta

 Birth of a Haemayopoietic Stem Cell in the Aorta of an embryonic mouse, full field of view. Confocal microscope, 40x dry lens (n.a. 0.6) pinhole 2 airy units, Z-range 125 µm in 5 µm steps, pixelsize 292x292 µm. Ext 488 (Ly6a-GFP) Em BP 500-550; Ext 561 (CD31-PE) Em 570-620. XYZ drift correction with the Stackreg plugin (P. Thévenaz, U.E. Ruttimann, M. Unser) in ImageJ (Wayne Rasband). Deconvolved with Huygens (SVI) deconvolution software. Jean-Charles Boisset,  Catherine Robin, Gert van Cappellen
 

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Download video Natures6

 
Birth of a Haemayopoietic Stem Cell in the Aorta of an embryonic mouse, cropped and rotated field of view. Confocal microscope, 40x dry lens (n.a. 0.6) pinhole 2 airy units, Z-range 125 µm in 5 µm steps, pixelsize 292x292 µm. Ext 488 (Ly6a-GFP) Em BP 500-550; Ext 561 (CD31-PE) Em 570-620. XYZ drift correction with the Stackreg plugin (P. Thévenaz, U.E. Ruttimann, M. Unser) in ImageJ (Wayne Rasband). Movie was showed in the 20h NOS journal. Deconvolved with Huygens (SVI) deconvolution software. Jean-Charles Boisset,  Catherine Robin, Gert van Cappellen