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SIM Microscopy

Super Resolution Wide Field Microscopy

Project started with two post-docs for two years in collaboration with the TU Delft to improve the structure illumination reconstruction algorithms and to startup biological experiments.Supervisors: Sjoerd Stallinga (TU Delft), Adriaan Houtsmuller and Gert van Cappellen (Erasmus MC)

 

Collaborations 3D Structured Illumination Microscopy

Chromatin structure and DNA damage:

 

Functional characterization of repair Foci during meiosis

Collaborators: Fabrizia Carofiglio, Willy Baarends

Department of Reproduction and Development, ErasmusMC Rotterdam

OIC: Maarten Paul *, Johan Slotman*, Gert van Cappellen*, Adriaan Houtsmuller*,

 

CTCF in DNA “kissing” events in Drosophila wing discs

Collaborators: Melanie Buxa, Rainer Renkawitz

Institute for Genetics, Justus-Liebig-Universität, Gießen

OIC: Martin van Royen *, Johan Slotman*, Adriaan Houtsmuller*

 

Neurology:

 

Ampa receptor GluR2 localization in purkinje cells

Collaborators: Zahnmin Lin, Zenyu Gao, Chris de Zeeuw

Department of neuroscience, ErasmusMC Rotterdam

OIC: Johan Slotman *, Maarten Paul*, Adriaan Houtsmuller

 

Vesicle docking in chromaffin cells

Collaborators: Girish KedarMatthijs Verhage

Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, VU University Amsterdam

OIC:  Johan Slotman *, Adriaan Houtsmuller*

 

Tubulin structure in neuromuscular junctions

Collaborator: Sreya Basu, Niels Galjart

Depatment of Cell Biology, ErasmusMC Rotterdam

OIC: Johan Slotman *, Gert van Cappellen*

 

Ampa Receptor interactome localization in synapses

Colaborators: Nikhil Pandya, Ka Wan Li, Guus Smit

Department of Molecular and Cellular Neurobiology, VU University Amsterdam

OIC: Johan Slotman *

 

Neuronal spine morphology in fragile X syndrome

Collaborators: Helen de Boer,  Rob Willemsen

Department of Clinical Genetics, ErasmusMC Rotterdam

OIC: Johan Slotman *, Gert-Jan Kremers*

 

Actin organization and extracellular matrix interactions:

 

Nanoscale organization of Focal Adhesions

OIC: Karin Legerstee **, Johan Slotman*, Adriaan Houtsmuller*

 

Adherence Junction organisation

Collaborators: Joppe Oldenburg, Johan de Rooij

Department of Molecular Cancer, UMC Utrecht

OIC: Johan Slotman *, Adriaan Houtsmuller*

 

Actin organisation within podosome clusters

Collaborators: Ben Joosten, Laurent Paardekoper, Allesandra Cambi

Tumor Immunology, NCMLS Nijmegen

OIC: Johan Slotman *, Adriaan Houtsmuller*

Other:

 

Fibril wound healing and NETtosis

Colaborators: Tamara Hoppenbrouwes, Monique de Maat, Han van Neck

Department of Plastic Surgery, Department of Hematology, ErasmusMC Rotterdam

OIC: Johan Slotman *, Tsion Abrahams*, Gert van Cappellen*

 

Structured Illumination Microscopy Development:

Advances in Reconstruction Algorithms and image analysis

Collaborators: Farzad Feridoeni, , Sjoerd Stallinga

Department of Imaging Physics, Delft University of Technology, Delft

OIC: Johan Slotman *, Gert van Cappellen*, Adriaan Houtsmuller*

 

Nanoscopy test and verification samples

Collaborators: Jacob Hoogenboom

Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft

OIC: Johan Slotman * , Gert van Cappellen* , Gert-Jan Kremers* , Adriaan Houtsmuller*

 

Publications:

Federici F, Mulugeta E, Schoenmakers S, Wassenaar E, Hoogerbrugge JW, van der Heijden GW, van Cappellen WA, Slotman JA, van IJcken WF, Laven JS, Grootegoed JA, Baarends WM (2015). Incomplete meiotic sex chromosome inactivation in the domestic dog.  BMC Genomics16:291.

 

Basu, S., S. Sladecek, H. Pemble, T. Wittmann, J. A. Slotman, W. van Cappellen, H. R. Brenner and N. Galjart (2014). "Acetylcholine receptor (AChR) clustering is regulated both by glycogen synthase kinase 3beta (GSK3beta)-dependent phosphorylation and the level of CLIP-associated protein 2 (CLASP2) mediating the capture of microtubule plus-ends." J Biol Chem 289(44): 30857-30867.

Righolt, C. H., J. A. Slotman, I. T. Young, S. Mai, L. J. van Vliet and S. Stallinga (2013). "Image filtering in structured illumination microscopy using the Lukosz bound." Opt Express 21(21): 24431-24451.

Oldenburg, J., G. van der Krogt, F. Twiss, A. Bongaarts, Y. Habani, J. A. Slotman, A. Houtsmuller, S. Huveneers and J. de Rooij (2015). "VASP, zyxin and TES are tension-dependent members of Focal Adherens Junctions independent of the alpha-catenin-vinculin module." Sci Rep5: 17225.

Panzer, L., L. Trube, M. Klose, B. Joosten, J. Slotman, A. Cambi and S. Linder (2015). "The formins FHOD1 and INF2 regulate inter- and intra-structural contractility of podosomes." J Cell Sci.

 

* Erasmus Optical Imaging Centre (OIC), ErasmusMC, Rotterdam

** Department of Pathology, ErasmusMC, Rotterdam