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Research project

An Integrated Tool-Kit for the Clinical Evaluation of Microbial Detection and Antibiotic Susceptibility Point-of-Care Testing Technologies (TEMPOtest-QC).

Status: Closed project

The TEMPOtest-QC project was an EU FP7-funded project that was established to help rapid diagnostic ‘point-of-care’ device developers for detecting respiratory and dental infections.

What we do

About our project

The TEMPOtest-QC project proposal addressed the need to “design integrated tools for closing the gap between microbiological point-of-care technologies and actual clinical need”, by designing, developing and implementing a microbiological point-of-care “tool-kit” comprising: i) a survey providing information on Europe-wide demand for point-of-care microbiological tests, ii) an archive of freely available quality control/quality assurance microbiological test samples for rapid evaluation of point-of-care microbiological tests, iii) a freely available Comparison Tool comprising specimen archive/software tool and iv) the optimization and development of electronic nose and antimicrobial resistance gene microarrays. TEMPOtest-QC logo

Our research focus

For more information see the Youtube video


1. Consultatie Implementatie Technisch Beheer B.V., Zuthphen, The Netherlands.

2. Universita’ Degli Studi Di Siena, Siena, Italy.

3. Universite Paris-Sud XI, Orsay, France. 

4. Universite Catholique de Louvain, Louvain-la-Neuve, Belgium.

5. Quality Control for Molecular Diagnostics, Glasgow, United Kingdom.


Perceptions of point-of-care infectious disease testing among European medical personnel, point-of-care test kit manufacturers, and the general public. Wendy E Kaman, Eleni-Rosalina Andrinopoulou, John P. Hays. Patient Preference and Adherence. 2013.  http://dx.doi.org./10.2147/PPA.S44889 

Emergence of multidrug-resistant NDM-1-producing Gram-negative bacteria in Bangladesh. M. A. Islam, P. K. Talukdar, A. Hoque, M. Huq, A. Nabi, D. Ahmed, K. A. Talukder, M. A. C. Pietroni, J. P. Hays, A. Cravioto and H. P. Endtz. European Journal of Clinical Microbiology and Infectious Diseases. 2012. 31(10):2593-600

Detection and Occurrence of Plasmid-Mediated AmpC in Highly Resistant Gram-Negative Rods. E. Ascelijn Reuland,  John P. Hays, Denise M. C. de Jongh, Eman Abdelrehim, Ina Willemsen, Jan A. J. W. Kluytmans, Paul H. M. Savelkoul, Christina M. J. E. Vandenbroucke-Grauls,  Nashwan al Naiemi.  PLOS ONE. 2014. 9(3): e91396. doi: 10.1371/journal.pone.0091396

Whole-genome mapping for high-resolution genotyping of Pseudomonas aeruginosa. S.A. Boers, R. Burggrave, M. van Westreenen, W.H.F. Goessens, J.P. Hays.  Journal of Microbiological Methods. 2014. Volume 106, November 2014, Pages 19–22. DOI: 10.1016/j.mimet.2014.07.020

The papain inhibitor (SPI) of Streptomyces mobaraensis inhibits bacterial cysteine proteases and is an antagonist of bacterial growth. Stephan Zindel, Wendy E. Kaman, Sabrina Fröls, Felicitas Pfeifer, Anna Peters, John P. Hays and Hans-Lothar Fuchsbauer.  2013. Antimicrobial Agents Chemotherapy. 2013. July vol. 57 no. 7 3388-3391. doi: 10.1128/AAC.00129-13

Evaluation of a FRET-peptide Substrate to Predict Virulence in Pseudomonas aeruginosa. Wendy E. Kaman, Nora El Arkoubi-El Arkoubi, Sanne Roffel, Hubert P. Endtz, Alex van Belkum, Floris J. Bikker, John P. Hays. PLOS ONE. 2013. DOI: 10.1371/journal.pone.0081428 

Highly specific protease-based approach for detection of Porphyromonas gingivalis in diagnosis of periodontitis. Kaman W, Galassic F,  de Soetd JJ, Bizzarroc S, Loosc BG, Veerman ECI, van Belkuma A, Hays JP and Bikker FJ. Journal of Clinical Microbiology. 2012. 50:104-12

Our team

Dr. Wendy Kaman w.e.kaman@acta.nl
Denise Vermeulen