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G. (Gisela) Ambagtsheer

Research Technician

  • Department
  • Surgery
  • Focus area
  • Machine perfusion, kidney transplantation



I am a senior research technician .I am interested in organ injury, inflammation and regeneration following surgical interventions such as organ transplantation.

We are studying how interventions such as dietary restriction and novel techniques such as machine perfusion may benefit patients using model systems, and translate these findings to human trials. 

Field(s) of expertise

In vitro and in vivo models, micro surgery

Education and career

2014- present: Senior Research technician, at the Laboratory for Experimental Transplantation and Intestinal Surgery (LETIS), Department of Surgery, Erasmus University Medical Center Rotterdam

2000 - 2014: Research technician, Laboratory for Experimental Surgical Oncology, Department of Surgery, Erasmus MC Rotterdam

1999-2000: Intern, Laboratory for Experimental Surgical Oncology, Department of Surgery, Erasmus MC Rotterdam


Fang Y, van Ooijen L, Ambagtsheer G, Nikolaev AV, Clahsen-van Groningen MC, Dankelman J, de Bruin RWF, Minnee RC. Real-time laser speckle contrast imaging measurement during normothermic machine perfusion in pretransplant kidney assessment. Lasers Surg Med. 2023 Oct;55(8):784-793.

Wijngaarden LH, Taselaar AE, Nuijten F, van der Harst E, Klaassen RA, Kuijper TM, Jongbloed F, Ambagtsheer G, Klepper M, IJzermans JNM, de Bruin RWF, Litjens NHR. T and B Cell Composition and Cytokine Producing Capacity Before and After Bariatric Surgery. Front Immunol. 2022 Jul 4;13:888278.

Willigenburg HV, Domburg BV, Ambagtsheer G, Brandt RM, Hesselink DA, de Bruin RW, de Winter BC. Validation of a dried blood spot method to measure tacrolimus concentrations in small volumes of mouse blood. Bioanalysis. 2022 Apr;14(7):441-449.

Wijngaarden LH, van der Harst E, Klaassen RA, Dunkelgrun M, Kuijper TM, Klepper M, Ambagtsheer G, IJzermans JNM, de Bruin RWF, Litjens NHR. Effects of Morbid Obesity and Metabolic Syndrome on the Composition of Circulating Immune Subsets. Front Immunol. 2021 Jul 20;12:675018. doi: 10.3389/fimmu.2021.675018. PMID: 34354700; PMCID: PMC8330422.

Levolger S, Wiemer EAC, van Vugt JLA, Huisman SA, van Vledder MG, van Damme- van Engel S, Ambagtsheer G, IJzermans JNM, de Bruin RWF. Inhibition of activin- like kinase 4/5 attenuates cancer cachexia associated muscle wasting. Sci Rep. 2019 Jul 8;9(1):9826. .

Levolger S, van den Engel S, Ambagtsheer G, IJzermans JNM, de Bruin RWF. Caloric restriction is associated with preservation of muscle strength in experimental cancer cachexia. Aging (Albany NY). 2018 Dec 26;10(12):4213-4223.

Jongbloed F, Saat TC, Verweij M, Payan-Gomez C, Hoeijmakers JH, van den Engel S, van Oostrom CT, Ambagtsheer G, Imholz S, Pennings JL, van Steeg H, IJzermans JN, Dollé ME, de Bruin RW. A signature of renal stress resistance induced by short-term dietary restriction, fasting, and protein restriction. Sci Rep. 2017 Jan 19;7:40901.

van Etten B, Eggermont AM, van Tiel ST, Ambagtsheer G, de Wilt JH, ten Hagen TL. Gene therapy in in vivo isolated perfusion models. Curr Gene Ther. 2005 Apr;5(2):195-202.


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