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Researcher

Dr. M.M. (Mathis) Funk

Researcher

Researcher

  • Focus area
  • Molecular biology, evolution, influenza polymerase, bioinformatics
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About

Introduction

Background

Mathis Funk (1990) is a post-doctoral researcher at the Viroscience department of Erasmus MC with a focus on hypothesis-driven fundamental research and viral evolution. He obtained his PhD in 2018 under the supervision of Thierry Heidmann for his work on syncytins, genes of viral origin that are necessary for placentation in animals. His results demonstrated for the first time that non-mammalian placentas also rely on genes of viral origin, suggesting that ancestral retroviral infections are a prerequisite for the emergence of placentation.

Current research

Dr. Funk joined the Viroscience department in 2019 to work on the evolution of influenza viruses, focusing on the molecular events leading to insertions and deletions in the influenza genome. Currently he is investigating the origin and mechanism of insertions leading to the emergence of highly pathogenic avian influenza viruses as well as new antigenically distinct human influenza B virus lineages. His work shifts the focus from traditional set ups to virus-free approaches focused on the molecular interplay of viral polymerase and genome.

Field(s) of expertise

Molecular biology, evolution, influenza polymerase, bioinformatics

Education and career

  • 2019-present: Post-doctoral researcher, Department of Viroscience, Erasmus MC, Rotterdam
  • 2014-2018: PhD in Virology, summa cum laude, Universite Paris-Sud (Paris, France)
  • 2014: Master of Science in molecular and medical virology, summa cum laude, Institut Pasteur (Paris, France) and Universite Pierre et Marie Curie (Paris France)
  • 2012-2013: One-year internship with Prof. Harald zur Hausen, Heidelberg, Germany
  • 2010-2014: Master of Science, Ecole Normale Superieure (Cachan, France) 
 

Publications

  • M. Funk, K. Gunst, V. Lucansky, H. Müller, H. Zur Hausen and E.-M. de Villiers, Isolation of protein-associated circular DNA from healthy cattle serum., Genome Announc. 2 (2014) 2014.; DOI:10.1128/genomeA.00846-14 
  • C. Whitley, K. Gunst, H. Müller, M. Funk, H. Zur Hausen and E.-M. de Villiers, Novel replication-competent circular DNA molecules from healthy cattle serum and milk and multiple sclerosis-affected human brain tissue., Genome Announc. 2 (2014) 4–5.; DOI:10.1128/genomeA.00849-14 
  • E.-M. de Villiers, M. Funk, K. Gunst, I. Lamberto Perez and H. Zur Hausen, HCBI sequences as an early marker for the future development of cancer and diseases of the CNS and as a target for cancer treatment and prevention. European patent No. EP3063298B1. Munich, Germany. European Patent Office. (2014) 
  • M. Funk*, G. Cornelis*, C. Vernochet, F. Leal, O. A. Tarazona, G. Meurice, O. Heidmann, A. Dupressoir, A. Miralles, M. P. Ramirez-Pinilla and T. Heidmann, An endogenous retroviral envelope syncytin and its cognate receptor identified in the viviparous placental Mabuya lizard, Proc. Natl. Acad. Sci. U. S. A. 114 (2017) E10991–E11000.; DOI:10.1073/pnas.1714590114 *contributed equally 
  • M. Funk, G. Cornelis, C. Vernochet, O. Heidmann, A. Dupressoir, A. Conley, S. Glickman and T. Heidmann, Capture of a hyena-specific retroviral envelope gene with placental expression associated in evolution with the unique emergence among carnivorans of hemochorial placentation in
  • P. Gultyaev, M. I. Spronken, M. Funk, R. A. M. Fouchier and M. Richard, Insertions of codons encoding basic amino acids in H7 hemagglutinins of influenza A viruses occur by recombination with RNA at hotspots near snoRNA binding sites, RNA 27 (2021) 123–132.; DOI:10.1261/rna.077495.120
 

Teaching activities

  • 2021: Group assignment for Immunity and Infection Master Summer course
  • 2014-2017: Practical and theoretical biology classes for 1st and 2nd year B.Sc students, Université Paris-Sud (Paris, France)
 

Scholarships, grants, and awards

Current

  • ZonMW Offroad grant (2020 round) to study the molecular mechanisms underlying insertion/deletion hotpsots in influenza virus. (EUR 100.000)

Past

  • 2018: PhD extension grant. 6-month grant by the French Foundation for Medical Research. (EUR 9000)
  • 2014-2017: Fellowship for PhD position. 3-year grant by the French Ministry of Education and research. Top 5%. (EUR 46.800)
  • 2014-2017: Teaching grant. 3-year by the French Ministry of Education and research. Top 5%. (EUR 7200)2010-2014: École Normale Supérieure scholarship. One of 60 French students to obtain a paid student position in 2010 after a nation-wide exam. (EUR 62.400)
 

Previous and current research

Identifying a new putative agent of human colon cancer

During my work in Prof. Zur Hausen’s lab, I investigated the hypothesis that the link between colon cancer in humans and red meat consumption is due to an unidentified viral pathogen by screening cattle sera for novel genomes. This led to the deposition of a patent using them as biomarkers in humans. These early discoveries have since been substantiated by the identification of numerous related genomes. The sequences I identified were the founding members of this new family of putative pathogens.

Expanding the role of Syncytins

When I started working on syncytins, it had already been well established that they were present all across the spectrum of placental mammals and that their fusiogenic ability was required for placental development and reproduction. What remained unclear was their role in other animal families as well as putative alternative roles in placental development. 
To tackle the first question, I investigated the case of a rare Andean placental lizard. Contrary to popular belief, placentation is not restricted to mammals and this lizard possesses a highly complex placenta, structurally very close to that of mammals. In collaboration with another PhD student, I was able to demonstrate that a syncytin was present at the placental level of the Andean lizard. This finally answered a long-standing question in the field and suggests that syncytins are not just an evolutive curiosity found in mammals, but that they may be present in many more animal families. It is also a stunning example of parallel evolution between mammals and lizards, whose evolution diverged about 300 million years ago. My work on the identification of the first non-mammalian syncytin was covered by the general press (The Economist, Le Monde) as well as the popular virology podcast “This Week in Virology”. More recently I was invited to speak as an expert on syncytins for the Netherlands Center for One Health Webinar “Viruses - Why we cannot live without them”.

A second project during my PhD concerned the placenta of Hyaenidae, which is strikingly different from all other carnivorans even though it presents the same carnivoran syncytin gene. Indeed, the Hyaenidae placenta is structurally and functionally much closer to human placentas. My investigations revealed the presence of another syncytin-like gene specifically in Hyaenidae placentas and adds to the growing list of syncytin-like genes in mammals, strongly hinting at the existence of more, as of yet unknown, roles for these genes.

Investigating the mechanism of influenza virus recombination events

Recombination is exceedingly rare in influenza viruses but has been shown to occur in some H7 avian influenza strains. These recombinations lead to the emergence of highly pathogenic avian influenza viruses (HPAIV) and seem to be guided or facilitated by snoRNAs binding close to the recombination site. Recombination in influenza viruses is still poorly understood. 
 

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