AMIE beeldmerk
Core Facility

AMIE

Applied Molecular Imaging Erasmus MC

AMIE provides a platform for scientists who share an interest in state-of-the-art imaging technology and molecular imaging assays for studying biological systems. AMIE's unique strength lies in the fusion of superb expertise in imaging modes, availability of exclusive mouse models, and its strong links between basic, translational and patient-bound research

Services

Methods / applications

Erasmus MC has been investing in imaging expertise and systems for quite some time and has built unique expertise in a number of areas. Various technologies (i.e. equipment as well as expertise) are put together in one new facility: Applied Molecular Imaging Erasmus. Combinations of imaging modalities can be applied to a single research question, or one experimental animal. From experience we know that a physical grouping of complementary techniques and expertise is a must to reach successful synergy. The new central imaging facility is largely housed in the new research unit in the Erasmus MC Experimental Animal Centre (EDC).

The research in the departments of Erasmus MC covers a wide range of topics; cancer research, microbiology/immunology, developmental biology and pharmacology.

Equipment

Core Equipment:

  • IVIS – Optical Imaging
  • FMT2500 – Optical Imaging
  • Quantum FX – microCT
  • VECTor – SPECT/PET, microCT and Optical Imaging
  • Inveon – PET
  • Discovery MR901 – 7T MRI
  • NanoScan – SPECT, 1T MRI
  • Vevo3100 - Ultrasound

 

Developmental/Other Equipment:

  • MSOT – Optoacoustic

Showcases

Name: Prof. dr. ir. Marion de Jong
Department:
Radiology & Nuclear Medicine
Project Title:
Imaging and therapy using the same radiolabelled molecules

General description of research:
An important research line of the Deptartment of Nuclear Medicine is the development and application of radiolabeled peptides and other tracers for tumor imaging (detection, staging and monitoring using PET and SPECT) and radionuclide therapy. These compounds can therefore detect cancer and treat it. The approach showed most convincing results: in the preclinical setting excellent tumoricidal effects were found, whereas in the clinic in patients suffering from neuroendocrine tumors > 75% response rate is achieved. In order to be able to detect and treat more tumor types, we now focus on other peptide receptor systems as well (e.g. CCK, minigastrin, bombesin, GRP analogues). A short youtube video showing the concept of our work can be found at: https://www.youtube.com/watch?v=0YvG_MCXZsw

SPECT-CT

PET_dynamics

Access & costs

Access to and use of the AMIE instrumentation is allowed under supervision of the AMIE. Training is provided for those who plan to use the equipment independently.

Support

The AMIE provides full-service support on all equipment for imaging experiments with the option to obtain training for unsupported work.

All imaging modalities have isoflurane equipment for anesthesia.

Training & education

Training

Users who would like to perform their own imaging research can be trained to use the equipment and make imaging protocols optimized for their research question, as well as training to analyze the data acquired.

Education

The AMIE regularly organized the AMIE translational imaging workshop to educate participants ranging from clinicians to PhD students interested in imaging research on the many ways preclinical imaging works and how it benefits research.

News, events & publications

News

AMIE recognized as core facility: 
https://nieuws.erasmusmc.nl/nieuws/nog-een-cofa-erkend-amie/

Maintenance NanoScan (SPECT-MRI) system 27- 31 January 2020.

 

Events

NKRV. 24 January 2020. http://www.nkrv.nl/

ISMRM - Benelux chapter. 24 January 2020. https://www.ismrm-benelux.org/

 

Molecular medicine day. March 18th 2020. www.molmed.nl

 

European Molecular Imaging Meeting. 24-27 March 2020. http://www.e-smi.eu/index.php?id=1976

 

Applied Molecular Imaging symposium. April 23rd 2020. www.erasmusmc.nl/amie

 

Translational Imaging Workshop by AMIE ‘From mouse to man’. June 2020. www.molmed.nl

 

European Association of Nuclear Medicine. Congress 17-21 October 2020. Vienna, Austria. https://www.eanm.org/congresses-events/future-congress/

 

Biomedical Research Techniques. October 2020. www.molmed.nl

Publications and theses

Publications

Imaging of atherosclerosis, targeting LFA-1 on inflammatory cells with 111In-DANBIRT. Meester EJ, Krenning BJ, de Blois RH, Norenberg JP, de Jong M, Bernsen MR, Van der Heiden K.

J Nucl Cardiol. 2019 Oct;26(5):1697-1704.

Imaging heterogeneity of peptide delivery and binding in solid tumors using SPECT imaging and MRI. Haeck JC, Bol K, de Ridder CM, Brunel L, Fehrentz JA, Martinez J, van Weerden WM, Bernsen MR, de Jong M, Veenland JF. EJNMMI Res. 2016 Dec;6(1):3.

Nanoparticles and clinically applicable cell tracking. Bernsen MR, Guenoun J, van Tiel ST, Krestin GP. Br J Radiol. 2015 Oct;88(1054):20150375.

Magnetic Resonance Detection of CD34+ Cells from Umbilical Cord Blood Using a 19F Label. Duinhouwer LE, van Rossum BJ, van Tiel ST, van der Werf RM, Doeswijk GN, Haeck JC, Rombouts EW, Ter Borg MN, Kotek G, Braakman E, Cornelissen JJ, Bernsen MR. PLoS One. 2015 Sep 22;10(9):e0138572.

In vivo quantitative assessment of cell viability of gadolinium or iron-labeled cells using MRI and bioluminescence imaging. Guenoun J, Ruggiero A, Doeswijk G, Janssens RC, Koning GA, Kotek G, Krestin GP, Bernsen MR. Contrast Media Mol Imaging. 2013 Mar-Apr;8(2):165-74.

Evaluation of a Fluorescent and Radiolabeled Hybrid Somatostatin Analog In Vitro and in Mice Bearing H69 Neuroendocrine Xenografts. Santini C, Kuil J, Bunschoten A, Pool S, de Blois E, Ridwan Y, Essers J, Bernsen MR, van Leeuwen FW, de Jong M.J Nucl Med. 2016 Aug;57(8):1289-95.

In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography. van Deel E, Ridwan Y, van Vliet JN, Belenkov S, Essers J. J Vis Exp. 2016 Feb 16;(108):53603.

 

THESIS

Preclinical SPECT-MRI; In vivo radiopeptide biodistribution studies. Joost Haeck, 2016

About us

Introduction

Imaging at the AMIE

The AMIE is a facility where pre-clinical imaging equipment is centrally located and accessible to investigators with a research question which can be answered using the specialist equipment under guidance of imaging experts.

Our team

Dr. Monique Bernsen – Head daily management

Dr. Jeroen Essers – Head innovation

BSc. Sandra van Tiel – Application specialist Nuclear Imaging & MRI

BSc. Yanto Ridwan – Application specialist Optical Imaging & CT

Dr. Joost Haeck – Application specialist MRI & Nuclear Imaging

Our expertise

AMIE is uniquely positioned within the Netherlands and relative to facilities in other countries worldwide. Not only does AMIE house an extensive array of imaging equipment with all modalities currently used for preclinical in vivo imaging, AMIE also has expert staff dedicated to the facility and is therefore able to continuously offer research support. Through close interaction with scientific experts and industry, AMIE fulfils a central role in the cycle of advancing imaging techniques, innovative research, and technology development, including hardware, software and wetware. That all equipment is only meters apart or even combined in a single platform is an immense benefit: study objects can be easily transferred between platforms giving investigators the opportunity to use several imaging modalities to gain extensive insight into their research question. This has already resulted in unique discoveries in various fields, e.g. stem-cell, cardiovascular and cancer research, which would not have been possible using single imaging modalities, and there remain many more applications which have yet to benefit from multi-modal experiments. Furthermore, imaging maximizes the information that can be gained from a given animal (refining), contributing to reduction of laboratory animal use.

The unique combination of extensive pre-clinical imaging equipment, cutting-edge technology, and dedicated in-house experts makes AMIE attractive to researchers both in NL and worldwide.

Collaboration

Erasmus MC departments using AMIE:

Experimental Cardiology

Molecular Genetics

Biomedical Engineering

Urology

Internal Medicine

Immunology

Pulmonology

Neurology

Pediatrics

Hematology

Rheumatology

Orthopedics

Virology

Medical Oncology

Pharmacology

Pulmonology

Molecular Radiation Therapy

(Experimental) Surgery

Radiology & Nuclear Medicine

 

Dutch academic users:

AMC, Dept of Vascular Disease (V. de Waard et al)

LUMC, Dept of Radiology (Dr Eic Kaijzel et al, Dr. Fijs van Leeuwen et al.; Dr Louise van de Weerd)

Radboud UMC, Dept of Radiology and Nuclear Medicine (Dr Frank. Nijssen)

Radboud UMC, Radboud Institute for Molecular Life Sciences (Dr Mangala Srinivas)

TU Delft, Reactor Institute (Dr Antonia Denkova et al.; Dr. Marlies Goorden et al., Prof. Freek Beekman et al.Dr Dennis Schaart et al.)

TU Delft, 3ME (Dr Sharam JanBaz)

UVA, Biomedical Engineering and Physics department (Dr Gustav Strijkers)

 

Dutch non-academic users:

Milabs, Inc

Cenya BV

Quirem BV

 

International academic users:

Gent University (J. de Backer et al),

University of Pittsburgh (M. Weinberg, Z. Urban)

Athens (Demokritos): Theodosia Maina-Nock et al.

Montpellier University (Faculty of Pharmacy): Prof. Jean Martinez  et al.

Bad Berka, Kantonsspital, Prof. Richard Baum et al.

 

International non-academic users:

AAA (Novartis), Inc