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AMIE beeldmerk
Core Facility

AMIE - Applied Molecular Imaging Erasmus MC

AMIE is a Core facility research with state-of-the-art imaging technology with dedicated management and expertise. AMIE offers various services: we are an accessible infrastructure, training center and expert center regarding preclinical and translational imaging techniques. A unique strength of the AMIE is that we harbor a wide plethora of imaging technologies in combination with strong ties to expert users, availability of exclusive disease models and established links between basic, translational and patient-bound research.

Services

Methods / applications

Erasmus MC has been investing in imaging expertise and systems for quite some time and has built unique expertise in a number of areas. Various technologies (i.e. equipment as well as expertise) are put together in one new facility: Applied Molecular Imaging Erasmus. Combinations of imaging modalities can be applied to a single research question, or one experimental animal. From experience we know that a physical grouping of complementary techniques and expertise is a must to reach successful synergy. The new central imaging facility is largely housed in the new research unit in the Erasmus MC Experimental Animal Centre (EDC).

The research in the departments of Erasmus MC covers a wide range of topics; cancer research, microbiology/immunology, developmental biology and pharmacology.

Equipment

Core Equipment:

  • IVIS – Optical Imaging
  • FMT2500 – Fluorescent Optical Imaging
  • Quantum FX – microCT
  • VECTor – SPECT/PET, microCT and Optical Imaging
  • Inveon – PET
  • Discovery MR901 – 7T MRI
  • NanoScan – SPECT, 1T MRI
  • Vevo3100 - Ultrasound (with LAZR-X photoacoustic addition)

 

Developmental/Other Equipment:

  • MSOT – Optoacoustic
  • Gammacounter for radioactive samples

Showcases

Name: Prof. dr. ir. Marion de Jong
Department:
Radiology & Nuclear Medicine
Project Title:
Imaging and therapy using the same radiolabelled molecules

General description of research:
An important research line of the Deptartment of Nuclear Medicine is the development and application of radiolabeled peptides and other tracers for tumor imaging (detection, staging and monitoring using PET and SPECT) and radionuclide therapy. These compounds can therefore detect cancer and treat it. The approach showed most convincing results: in the preclinical setting excellent tumoricidal effects were found, whereas in the clinic in patients suffering from neuroendocrine tumors > 75% response rate is achieved. In order to be able to detect and treat more tumor types, we now focus on other peptide receptor systems as well (e.g. CCK, minigastrin, bombesin, GRP analogues). A short youtube video showing the concept of our work can be found at: https://www.youtube.com/watch?v=0YvG_MCXZsw

SPECT-CT

PET_dynamics

Access & costs

Access to and use of the AMIE instrumentation is allowed under supervision of the AMIE. Training is provided for those who plan to use the equipment independently.

 

Costs for equipment depends on user requirements. For more information contact the AMIE.

Support

The AMIE provides full-service support on all equipment for imaging experiments with the option to obtain training for unsupported work.

All imaging modalities have isoflurane equipment for anesthesia.

Training & education

Training

Users who would like to perform their own imaging research can be trained to use the equipment and make imaging protocols optimized for their research question, as well as training to analyze the data acquired.

Education

The AMIE translational imaging workshop aims to educate participants ranging from clinicians to PhD students interested in imaging research on the many ways preclinical imaging works and how it benefits research. The next workshop will be held from 16 to 18 November. The preliminary program is shown below and is subject to changes.

Day 1 – Tuesday 16 November

Moderator:

Time

Title

Speaker

09:00 - 09:30

Registration

09:30 - 09:40

Welcome and short introduction to the Translational Imaging Workshop

Monique Bernsen

Erasmus MC–AMIE Core Facility

09:40 - 10:20

Nuclear (PET; SPECT) Imaging - Basic Principles

Marlies Goorden

TU Delft – Biomedical Imaging

10:20 - 11:00

Nuclear Imaging- Applications

Sophia Koustoulidou

Erasmus MC–Radiology & Nuclear Medicine

11:00 - 11:15

Coffee break

11:15 - 12:05

Practical aspects of Nuclear Imaging

Sandra van Tiel

Erasmus MC–AMIE Core Facility

12:05 - 12:45

MRI - Basic Principles

Gustav Strijkers

Amsterdam UMC-Biomedical Engineering and Physics

12:45 - 14:00

Lunch & Technical exhibit (companies)

14:00 - 14:45

MRI - Applications

Uwe Himmelreich

KU Leuven - Biomedical MRI

14:45 - 15:35

Practical aspects of MR Imaging

 

Joost Haeck

Erasmus MC–AMIE Core Facility

15:35 - 15:50

Coffee break

 

15:50 - 16:50

Keynote speaker Translational Imaging

Edwin Oei

Erasmus MC–Radiology & Nuclear Medicine

16:50 - 17:45

Tour AMIE facility

AMIE team

Erasmus MC-AMIE Core Facility

18:00 - 21:00

Diner

T.B.D.

 

Day 2 – Wednesday 17 November

Moderator:

Time

Title

Speaker

09:00 - 09:40

microCT - Basic Principles

Marcel van Straten

Erasmus MC-Radiology & Nuclear Medicine

09:40 - 10:20

microCT- Applications

Sasha Belenkov

Perkin Elmer

10:20 - 11:00

Coffee break

11:00 - 11:50

Practical aspects of microCT imaging

 

Yanto Ridwan

Erasmus MC-AMIE Core Facility

11:40 - 12:20

Fluorescent imaging - Basic Principles & Applications

Clemens Lowik

Erasmus MC-Radiology & Nuclear Medicine

12:20 - 13:30

Lunch & Technical exhibit (companies)

13:30 - 14:10

Bioluminescent imaging - Basic Principles & Applications

Laura Mezzanotte

Erasmus MC-Radiology & Nuclear Medicine

14:10 - 15:00

Practical aspects of optical imaging

 

Yanto Ridwan

Erasmus MC-AMIE Core Facility

15:00 - 15:15

Coffee break

 

15:15 - 16:15

Keynote speaker(s) Translational Imaging

Stijn Keereweer

Erasmus MC-Ear, nose and throat medicine

16:15 - 17:15

Tour AMIE facility

AMIE Team

Erasmus MC-AMIE Core Facility

 

Day 3 - Thursday 18 November

Moderator:

Time

Title

Speaker

09:00 - 09:40

Ultrasound imaging - Basic Principles & Applications

Hans Bosch

Erasmus MC- Biomedical Engineering

09:40 - 10:20

Ultrasound molecular imaging - Basic Principles & Applications

Klazina Kooiman

Erasmus MC- Biomedical Engineering

10:20 - 10:40

Coffee break

10:40 - 11:20

Practical aspects of Ultrasound Imaging

TBD&

11:20 - 12:00

Photoacoustics - Basic Principles

Anton Nikolaev

Erasmus MC- Biomedical Engineering

12:00 - 13:00

Lunch & Technical exhibit (companies)

13:00 - 13:30

Photoacoustics - Applications

Kranthi Panth

Erasmus MC-AMIE Core Facility/

Molecular Genetics

13:30 - 14:00

Practical aspects of photoacoustic imaging

Joost Haeck

Erasmus MC-AMIE Core Facility

14:00 - 14:15

Coffee break

 

14:15 - 15:55

Multimodal imaging: Opportunities and Challenges

T.B.A.

MILabs

15:55 - 16:55

Keynote speaker(s) Translational Imaging

Gijs van Soest

Erasmus MC- Biomedical Engineering

16:55 - 17:10

Closing

Monique Bernsen

Erasmus MC-AMIE Core Facility


News, events & publications

News

New multimodal phantom acquired:

Bioemtech fillable phantom for multimodal mouse imaging

 

 

 

 

 

 

 

Events


Publications and theses

Publications

Nuclear Imaging Post-infarction Inflammation in Ischemic Cardiac Diseases - New Radiotracers for potential Clinical Applications. Krenning BJ, der Heiden KV, Duncker DJ, de Jong M, Bernsen MR.Curr Radiopharm. 2020 Oct 12.

Computed Tomography and Magnetic Resonance Imaging.
Bernsen MR, van Straten M, Kotek G, Warnert EAH, Haeck JC, Ruggiero A, Wielopolski PA, Krestin GP. Recent Results Cancer Res. 2020;216:31-110.

Imaging of inflammatory cellular protagonists in human atherosclerosis: a dual-isotope SPECT approach.
Barrett HE, Meester EJ, van Gaalen K, van der Heiden K, Krenning BJ, Beekman FJ, de Blois E, de Swart J, Verhagen HJ, Maina T, Nock BA, Norenberg JP, de Jong M, Gijsen FJH, Bernsen MR.Eur J Nucl Med Mol Imaging. 2020 Nov;47(12):2856-2865.

Imaging of atherosclerosis, targeting LFA-1 on inflammatory cells with 111In-DANBIRT. Meester EJ, Krenning BJ, de Blois RH, Norenberg JP, de Jong M, Bernsen MR, Van der Heiden K. 
J Nucl Cardiol. 2019 Oct;26(5):1697-1704.

Imaging heterogeneity of peptide delivery and binding in solid tumors using SPECT imaging and MRI. Haeck JC, Bol K, de Ridder CM, Brunel L, Fehrentz JA, Martinez J, van Weerden WM, Bernsen MR, de Jong M, Veenland JF. EJNMMI Res. 2016 Dec;6(1):3.

Nanoparticles and clinically applicable cell tracking. Bernsen MR, Guenoun J, van Tiel ST, Krestin GP. Br J Radiol. 2015 Oct;88(1054):20150375.

Magnetic Resonance Detection of CD34+ Cells from Umbilical Cord Blood Using a 19F Label. Duinhouwer LE, van Rossum BJ, van Tiel ST, van der Werf RM, Doeswijk GN, Haeck JC, Rombouts EW, Ter Borg MN, Kotek G, Braakman E, Cornelissen JJ, Bernsen MR. PLoS One. 2015 Sep 22;10(9):e0138572.

In vivo quantitative assessment of cell viability of gadolinium or iron-labeled cells using MRI and bioluminescence imaging. Guenoun J, Ruggiero A, Doeswijk G, Janssens RC, Koning GA, Kotek G, Krestin GP, Bernsen MR. Contrast Media Mol Imaging. 2013 Mar-Apr;8(2):165-74.

Evaluation of a Fluorescent and Radiolabeled Hybrid Somatostatin Analog In Vitro and in Mice Bearing H69 Neuroendocrine Xenografts. Santini C, Kuil J, Bunschoten A, Pool S, de Blois E, Ridwan Y, Essers J, Bernsen MR, van Leeuwen FW, de Jong M.J Nucl Med. 2016 Aug;57(8):1289-95.

In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography. van Deel E, Ridwan Y, van Vliet JN, Belenkov S, Essers J. J Vis Exp. 2016 Feb 16;(108):53603.

 

THESIS

Preclinical SPECT-MRI; In vivo radiopeptide biodistribution studies. Joost Haeck, 2016

About us

Introduction

Imaging at the AMIE

The AMIE is a facility where pre-clinical imaging equipment is centrally located and accessible to investigators with a research question which can be answered using the specialist equipment under guidance of imaging experts.

 

For inquiries please send an email to amie@erasmusmc.nl

Our team

Dr. Monique Bernsen has a PhD in Medical Biology and has been active in Molecular Imaging since 2004. In 2010  she was involved as co-chair in the realization of the AMIE facility, and since 2019 she is Head Operations and Daily Management of the AMIE Core Facility. You can always contact her for questions about the possibilities that AMIE offers for your research and for entering into collaborations. Our goal is to promote and enable the use of advanced preclinical imaging techniques in any way possible.

Dr. Jeroen Essers holds a PhD in molecular biology and is a group leader Molecular Imaging at the Erasmus MC. As Head Innovation of the AMIE Core facility he wants to strengthen the further development of medical and advanced imaging technology and he therefore focusses on exploring, recommending and designing new imaging modalities and programs in order to maintain the pioneering role of the AMIE imaging facility. This way we established a knowledge centre for imaging technology and an accessible test location for new imaging technology. Start-ups can develop innovative imaging technology and present themselves more easily to potential users and investors. Innovation and collaboration are achieved through ease of accessibility of AMIE for internal and external partners.

Dr. Sandra van Tiel completed a BSc in medical biology (1995) at the Hoger Laboratorium Onderwijs in Delft and a PhD in cellular imaging (2020) at the ErasmusMC in Rotterdam. Sandra was appointed to her current position in April 2019. Her work primarily involves performing and supervising imaging experiments using PET, SPECT, CT and MRI. Sandra is the registered coordinating radiation expert and the animal coordinator of the AMIE. You can contact her if you need help setting up your experiment, but also if you need help writing a study protocol or project license.

Yanto Ridwan completed a BSc in Medical Biology (2007) at the Hoger Laboratorium Onderwijs in Rotterdam. He was appointed as an application specialist, with the main focus on optical imaging and microCT. His tasks include providing preliminary advice regarding the use of preclinical imaging modalities and he also provides technical support, application development, education, planning and maintenance of equipment.

Dr. Joost Haeck completed his PhD in molecular imaging in 2016 at the Erasmus MC. Since then he has been active in the AMIE as coordinator and application specialist. From the inclusion of AMIE as Core Facility Joost has focused on the role of application specialist on the MRI and Nuclear imaging equipment as well as coordinator of data and general IT needs of the AMIE.

Our expertise

AMIE is uniquely positioned within the Netherlands and relative to facilities in other countries worldwide. Not only does AMIE house an extensive array of imaging equipment with all modalities currently used for preclinical in vivo imaging, AMIE also has expert staff dedicated to the facility and is therefore able to continuously offer research support. Through close interaction with scientific experts and industry, AMIE fulfils a central role in the cycle of advancing imaging techniques, innovative research, and technology development, including hardware, software and wetware. That all equipment is only meters apart or even combined in a single platform is an immense benefit: study objects can be easily transferred between platforms giving investigators the opportunity to use several imaging modalities to gain extensive insight into their research question. This has already resulted in unique discoveries in various fields, e.g. stem-cell, cardiovascular and cancer research, which would not have been possible using single imaging modalities, and there remain many more applications which have yet to benefit from multi-modal experiments. Furthermore, imaging maximizes the information that can be gained from a given animal (refining), contributing to reduction of laboratory animal use.

The unique combination of extensive pre-clinical imaging equipment, cutting-edge technology, and dedicated in-house experts makes AMIE attractive to researchers both in NL and worldwide.

Collaboration

Erasmus MC departments using AMIE:

Experimental Cardiology

Molecular Genetics

Biomedical Engineering

Urology

Internal Medicine

Immunology

Pulmonology

Neurology

Pediatrics

Hematology

Rheumatology

Orthopedics

Virology

Medical Oncology

Pharmacology

Pulmonology

Molecular Radiation Therapy

(Experimental) Surgery

Radiology & Nuclear Medicine

 

Dutch academic users:

AMC, Dept of Vascular Disease (V. de Waard et al)

LUMC, Dept of Radiology (Dr Eic Kaijzel et al, Dr. Fijs van Leeuwen et al.; Dr Louise van de Weerd)

Radboud UMC, Dept of Radiology and Nuclear Medicine (Dr Frank. Nijssen)

Radboud UMC, Radboud Institute for Molecular Life Sciences (Dr Mangala Srinivas)

TU Delft, Reactor Institute (Dr Antonia Denkova et al.; Dr. Marlies Goorden et al., Prof. Freek Beekman et al.Dr Dennis Schaart et al.)

TU Delft, 3ME (Dr Sharam JanBaz)

UVA, Biomedical Engineering and Physics department (Dr Gustav Strijkers)

 

Dutch non-academic users:

Milabs, Inc

Cenya BV

Quirem BV

 

International academic users:

Gent University (J. de Backer et al),

University of Pittsburgh (M. Weinberg, Z. Urban)

Athens (Demokritos): Theodosia Maina-Nock et al.

Montpellier University (Faculty of Pharmacy): Prof. Jean Martinez  et al.

Bad Berka, Kantonsspital, Prof. Richard Baum et al.

 

International non-academic users:

AAA (Novartis), Inc

Career opportunities

No positions currently available