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Research group/lab  |  P.I. T.S. Barakat, MD PhD

Barakat lab: Non-Coding Genome in Clinical Genetics

Our team focusses on deciphering the role of the non-coding genome in neurodevelopmental disorders and human embryonic stem cells

About our research group/lab

Our research

The non-coding genome

We know that most DNA sequences (~98%) in the human genome do not encode protein-coding genes. Yet, our understanding of those sequences and why they are important is still incomplete. An important group of non-coding genome elements are enhancers, which are crucial for the proper regulation of spatiotemporal gene expression. Although techniques such as ChIP-seq, chromatin accessibility assays, etc. can predict the identity of enhancers, predicting the activity of these sequences is still difficult. Understanding the non-coding genome is highly relevant, as many cases of genetic disorders are currently unexplained. A large fraction of this missing heritability is likely caused by aberrations in non-coding sequences.

New technologies to study the non-coding genome

Our newly-developed approach that combines chromatin immunoprecipitation with a massively parallel reporter assay allows generation of comprehensive, genome-wide enhancer activity maps for various cell types. This work has generated one of the largest resources of functionally validated enhancers in human embryonic stem cells existing to date (available at http://hesc-enhancers.computational-epigenetics.org), which will enable further knowledge-based studies to decipher the code underlying the gene regulation by non-coding sequences. Using these data, we discovered that only a small fraction of genomic regions bound by transcription factors or marked by histone modifications generally believed to be correlated to enhancers, show measurable enhancer activity in human embryonic stem cells. Active sites show a distinct protein binding profile and enrichment for sequences derived from transposable elements. Enhancer activity changes dramatically upon developmental transitions during differentiation, and only small constituents of “super-enhancers” are responsible for enhancer activity.   

Our current work 

We focus on deciphering the role of the non-coding genome in genetic disorders –particularly brain disorders. Using functional genomics and various other approaches, including induced pluripotent stem cells and cerebral organoids (“mini-brains”) and computational data mining, we are studying the enhancer landscape in cells representing neurodevelopment. We hypothesize that many genetic disorders of brain development are caused by alterations of non-coding elements, including enhancers. To directly test this hypothesis, we sequence enhancers identified through our genome-wide functional enhancer activity assays in patients. We are applying disease modelling for MCD neurodevelopmental disorder patients harboring identified mutations, using patient-specific induced pluripotent stem cells and differentiation to cerebral organoids, thereby simulating brain development in a dish. Our studies will help identify functional enhancer sequences in the non-coding genome, and will allow novel innovative approaches for patient diagnostics using functional genomics.
 
A novel research line focusses on identifying genetic causes of epileptic encephalopathy, a group of severe disorders presenting in early infancy with untreatable epilepsy and severe developmental delay. We recently discovered a new type of epileptic encephalopathy, caused by mutations in the UGP2 gene. This identified nucleotide sugar metabolism as an important pathway in this disease. We are working on methods to influence this pathway as possible future treatments. This new disease has recently been recognized as “Barakat-Perenthaler syndrome” by OMIM

We also discovered another previously unrecognized neurodevelopmental disorder caused by mutations in YIF1B. Patients with this disease have severe neurodevelopmental delay, movement disorders and often epilepsy. OMIM now refers to this disease as Kaya-Barakat-Masson syndrome.

Key Publications

Comprehensive multi-omics integration identifies differentially active enhancers during human brain development with clinical relevance.Yousefi S, Deng R, Lanko K, Salsench EM, Nikoncuk A, van der Linde HC, Perenthaler E, van Ham TJ, Mulugeta E#, Barakat TS# corresponding authors.Genome Medicine 13, 162 (2021).

Two new cases of chromosome 12q14 deletions and review of the literature.
 Ruizhi Deng*, Melysia T. McCalman*, Thomas P. Bossuyt, Tahsin Stefan Barakat # (2021). *shared first author; #corresponding author. Frontiers in Genetics.

The non-coding genome in genetic brain disorders: new targets for therapy?
Essays in Biochemistry. Eva Medico Salsench, Faidra Karkala, Kristina Lanko#, Tahsin Stefan Barakat# (2021) . 2021 Aug 20:EBC20200121. doi: 10.1042/EBC20200121 #corresponding authors.

Expanding the mutational landscape and clinical phenotype of the YIF1B related brain disorder. 
Brain. Eva Medico Salsench*, Reza Maroofian,*, Ruizhi Deng, Kristina Lanko, Anita Nikoncuk, Belén Pérez, Obdulia Sánchez-Lijarcio, Salvador Ibáñez-Mico, Antonina Wojcik, Marcelo Vargas, Nouriya Abbas Al-Sannaa, Marian Y. Girgis, Tainá Regina Damaceno Silveira, Peter Bauer, Audrey Schroeder, Chin-To Fong, Amber Begtrup, Meisam Babaei, Mehran Beiraghi Toosi, Farah Ashrafzadeh, Shima Imannezhad, Mohammad Doosti, Najmeh Ahangari, Paria Najarzadeh Torbati, Ehsan Ghayoor Karimiani, David Murphy, Elisa Cali, Ibrahim H Kaya, Mohammad AlMuhaizea, Dilek Colak, Cardona-Londoño KJ, Arold ST, Henry Houlden, Aida Bertoli-Avella, Namik Kaya#, Tahsin Stefan Barakat, # (2021) , *shared first author; #shared corresponding author.

Delineating the molecular and phenotypic spectrum of the SETD1B-related syndrome.
Genetics in Medicine. Weerts*, MJA, Lanko*, K, Guzman-Vega, FJ, Jackson, A, Ramakrishnan, R, Cardona-Londono, KJ, Pena-Guerra, KA, van Bever, Y, van Paassen, B, Kievit, A, van Slegtenhorst, M, Allen, NM, Kehoe, CM, Robinson, HK, Pang, L, Banu, SH, Zaman, M, Efthymiou, S, Houlden, H, Jarvela, I, Lauronen, L, Maatta, T, Schrauwen, I, Leal, SM, Ruivenkamp, CAL, Barge-Schaapveld, DQCM, Peeters-Scholte, CMPCD, Galehdari, H, Mazaheri, N, Sisodiya, SM, Harrison, V, Sun, A, Thies, J, Pedroza, LA, Taranchenko, YL, Chinn, IK, Lupski, JR, Garza-Flores, A, McGlothlin, J, Yang, L, Huang, S, Wang, X, Jewett, T, Rosso, G, Lin, X, Mohammed, S, Merritt, JL, Mirzaa, GM, Timms, AE, Scheck, J, Elting, M, Polstra, AM, Schenck, L, Ruzhnikov, MRZ, Vetro, A, Montomoli, M, Guerrini, R, Koboldt, DC, Mosher, TM, Pastore, MT, McBride, KL, Peng, J, Pan, Z, Willemsen, M, Koning, S, Turnpenny, PD, de Vries, BBA, Gilissen, C, Pfundt, R, Lees, M, Braddock, SR, Klemp, KC, Vansenne, F, van Gijn, M, Quindipan, C, Deardorff, MA, Hamm, JA, Putnam, AM, Baud, R, Walsh, L, Lynch, SA, Baptista, J, Person, RE, Monaghan, KG, Crunk, A, Keller-Ramey, J, Reich, A, Elloumi, HZ, Alders, M, Kerkhof, J, McConkey, H, Haghshenas, S, Genomics England Research Consortium, Maroofian, R, Sadikovic, B, Banka, S, Arold, ST, Barakat, TS# (2021) , 2021, Aug 3. doi: 10.1038/s41436-021-01246-2 . *shared first author; #corresponding author.

Models behind the mystery of establishing enhancer-promoter interactions.
European Journal of Cell Biology. Kathryn Monfils and Tahsin Stefan Barakat,# (2021100 (2021) 151170, doi.org/10.1016/j.ejcb.2021.151170; #corresponding author.

PIGG variant pathogenicity assessment reveals novel features within nineteen families. 
Genetics in Medicine Camille Tremblay-Laganière, Reza Maroofian, Thi Tuyet Mai Nguyen, Ehsan Ghayoor Karimiani, Salman Kirmani, Fizza Akbar, Shahnaz Ibrahim, Bushra Afroze, Mohammad Doosti, Farah Ashrafzadeh, Meisam Babaei, Stephanie Efthymiou, Marilena Christoforou, Tipu Sultan, Roger L. Ladda, Heather M. McLaughlin, Rebecca Truty, Sonal Mahida, Julie S. Cohen, Kristin Baranano, Fatima Y. Ismail, Millan S. Patel, Anna Lehman, Andrew C. Edmondson, Amanda Nagy, Melissa A. Walker, Saadet Mercimek-Andrews, Yuta Maki, Rani Sachdev, Rebecca Macintosh, Elizabeth E. Palmer, Grazia M.S. Mancini, Tahsin Stefan Barakat, Robert Steinfeld, Christina T. Rüsch, Georg M. Stettner, Matias Wagner, Saskia B. Wortmann, Usha Kini, Angela F. Brady, Karen L. Stals, Naila Ismayilova, Sian Ellard, Danilo Bernardo, Kimberly Nugent, Scott D. McLean, Stylianos E. Antonarakis, Henry Houlden, Taroh Kinoshita, Philippe M Campeau, Yoshiko Murakami 2021 Jun 10. doi: 10.1038/s41436-021-01215-9.

Bi-allelic VPS16 mutations reducing HOPS/CORVET complexes cause a mucopolysaccharidosis-like disease.
EMBO Molecular Medicine. Kalliopi Sofou *, Kolja Meier*, Leslie E. Sanderson*, Debora Kaminsk, Laia Montoliu Gaya, Emma Samuelsson, Maria Blomqvist, Lotta Agholme, Jutta Gärtner, Chris Mühlhausen, Niklas Darin, Tahsin Stefan Barakat, Lars Schlotawa, Tjakko van Ham, Jorge Asin Cayuela and Fredrik H. Sterky (2021), 2021, e13376.

Defects in KCNJ16 cause a novel tubulopathy with hypokalemia, salt wasting, disturbed acid-base homeostasis, and sensorineural deafness.
Journal of the American Society of Nephrology. Karl P Schlingmann*, Aparna Renigunta*, Ewout J Hoorn*, Anna-Lena Forst, Vijay Renigunta, Velko Athanasov, Sinthura Mahendran, Tahsin Stefan Barakat, Valentine Gillion, Nathalie Godefroid, Alice S Brooks, Dorien Lugtenberg, Jennifer Lake, Huguette Debaix, Christoph Rudin, Bertrand Knebelmann, Stephanie Tellier, Caroline Rousset-Rouviere, Daan Viering, Jeroen HF deBaaij, Stefanie Weber, Oleg Palygin, Alexander Staruschenko, Robert Kleta, Pascal Houillier, Detlef Bockenhauer, Rosa Vargas-Poussou, Olivier Devuyst, Richard Warth*, Anselm A Zdebik*, and Martin Konrad* (2021) “2021 Apr 2:ASN.2020111587. doi: 10.1681/ASN.2020111587.

Bi-allelic variants in the HOPS complex subunit VPS41 cause cerebellar ataxia and abnormal membrane trafficking.
Brain. Leslie E. Sanderson*, Kristina Lanko*, Maysoon Alsagob*, Rawan Almass*, Nada Al-Ahmady*, Maryam Najafi, Mohammad A. Al-Muhaizea, Hamad Alzaidan, Hesham AlDhalaan, Elena Perenthaler, Herma C. van der Linde, Anita Nikoncuk, Nicolas Kühn, Dinu Antony, Tarek Mustafa Owaidah, Salmo Raskin, Luana Gabriela Dalla Rosa Vieira, Romulo Mombach, Najmeh Ahangari, Tainá Regina Damaceno Silveira, Najim Ameziane, Arndt Rolfs, Aljohara Alharbi, Raghda M. Sabbagh, Khalid AlAhmadi, Bashayer Alawam, Hazem Ghebeh, Aljouhra AlHargan, Anoud A. Albader, Faisal S. Binhumaid, Ewa Goljan, Dorota Monies, Osama M. Mustafa, Mazhor Aldosary, Albandary AlBakheet, Banan Alyounes,Faten Almutairi, Ali Al-Odaib,Durdane Bekar Aksoy, A. Nazli Basak, Robin Palvadeau, Daniah Trabzuni, Jill A. Rosenfeld, Ehsan Ghayoor Karimiani, Brian Meyer, Bedri Karakas, Futwan Al-Mohanna, Stefan T. Arold, Dilek Colak, Reza Maroofian, Henry Houlden, Aida M. Bertoli-Avella, Miriam Schmidts,#, Tahsin Stefan Barakat#, Tjakko J. van Ham#, Namik Kaya#. 2021 Mar 25;awaa459. doi: 10.1093/brain/awaa459 *shared first author; #shared last senior and corresponding author.

SPEN haploinsufficiency causes a neurodevelopmental disorder overlapping proximal 1p36 deletion syndrome and is associated with a distinctive episignature of the X chromosome in females.
The American Journal of Human Genetics. Francesca Clementina Radio, Kaifang Pang, Andrea Ciolfi, Michael A. Levy, Andrés Hernández-García, Lucia Pedace, Francesca Pantaleoni, Zhandong Liu, Elke de Boer, Adam Jackson, Alessandro Bruselles, Haley McConkey, Emilia Stellacci, Stefania Lo Cicero, Marialetizia Motta, Rosalba Carrozzo, Maria Lisa Dentici, Kirsty McWalter, Megha Desai, Kristin G. Monaghan, Aida Telegrafi, Christophe Philippe, Antonio Vitobello, Margaret Au, Katheryn Grand, Pedro A. Sanchez-Lara, Joanne Baez, Kristin Lindstrom, Peggy Kulch, Jessica Sebastian, Suneeta Madan-Khetarpal, Chelsea Roadhouse, Jennifer J. MacKenzie, Berrin Monteleone, Carol J. Saunders, July K. Jean Cuevas, Laura Cross, Dihong Zhou, Taila Hartley, Sarah L. Sawyer, Fabíola Paoli Monteiro, Tania Vertemati Secches, Fernando Kok, Laura E. Schultz-Rogers, Erica L. Macke, Eva Morava, Eric W. Klee, Jennifer Kemppainen, Maria Iascone, Angelo Selicorni, Romano Tenconi, David J. Amor, Lynn Pais, Lyndon Gallacher, Peter Turnpenny, Karen Stals, Sian Ellard, Sara Cabet, Gaetan Lesca, Joset Pascal, Katharina Steindl, Sarit Ravid, Karin Weiss, Alison M.R. Castle, Melissa T. Carter, Louisa Kalsner, Bert B.A. de Vries, Bregje W. van Bon, Marijke R. Wevers, Rolph Pfundt, Alexander P.A. Stegmann, Bronwyn Kerr, Helen M. Kingston, Kate E. Chandler, Willow Sheehan, Abdallah F. Elias, Deepali N. Shinde, Meghan C. Towne, Nathaniel H. Robin, Dana Goodloe, Adeline Vanderver, Omar Sherbini, Krista Bluske, R. Tanner Hagelstrom, Caterina Zanus, Flavio Faletra, Luciana Musante, Evangeline C. Kurtz-Nelson, Rachel K. Earl, Britt-Marie Anderlid, Gilles Morin, Marjon van Slegtenhorst, Karin E.M. Diderich, Alice S. Brooks, Joost Gribnau, Ruben G. Boers, Teresa Robert Finestra, Lauren B. Carter, Anita Rauch, Paolo Gasparini, Kym M. Boycott, Tahsin Stefan Barakat, John M. Graham Jr., Laurence Faivre, Siddharth Banka, Tianyun Wang, Evan E. Eichler, Manuela Priolo, Bruno Dallapiccola, Lisenka E.L.M. Visser, Bekim Sadikovic, Daryl A. Scott, Jimmy Lloyd Holder Jr., Marco Tartaglia (2020) 2021 Feb 9:S0002-9297(21)00015-X. doi: 10.1016/j.ajhg.2021.01.015.

Isobutyryl-CoA dehydrogenase deficiency associated with autism in a girl without an alternative genetic diagnosis by trio whole exome sequencing: a case report.
Molecular Genetics and Genomics Medicine. Maria Eleftheriadou, Evita Medici- van den Herik, Kyra Stuurman, Yolande van Bever, Debby Hellebrekers, Marjon van Slegtenhorst, George Ruijter, Tahsin Stefan Barakat* (2020),2021;00:e1595. *corresponding author.

 

Deciphering the premature mortality in PIGA-CDG – an untold story.
Allan Bayat, Marius Kløvgaard, Katrine Johannesen, Tahsin Stefan Barakat, Anneke Kievit, Martino Montomoli, Elena Parrini, Nicola Pietrafusa, Helenius Jurgen Schelhaas, Marjon van Slegtenhorst, Kazushi Miya, Renzo Guerrini, Lisbeth Tranebjærg, Zeynep Tümer, Guido Rubboli, Rikke Møller Epilepsy Research.

BICRA, a SWI/SNF complex member, is associated with BAF-disorder related phenotypes in humans and model organism
Scott Barish*, Tahsin Stefan Barakat*, Brittany C. Michel*, Nazar Mashtalir*, Jennifer Phillips, Alfredo M. Valencia, Berrak Ugur, Jeremy Wegner, Tiana M. Scott, Brett Bostwick, Undiagnosed Diseases Network, David R. Murdock, Hongzheng Dai, Elena Perenthaler, Anita Nikoncuk, Marjon van Slegtenhorst, Alice S. Brooks, Boris Keren, Caroline Nava, Cyril Mignot, Jessica Douglas, Lance Rodan, Catherine Nowak, Sian Ellard, Karen Stals, Sally Ann Lynch, Marie Faoucher, Gaetan Lesca, Patrick Edery, Kendra L Engleman, Dihong Zhou, Isabelle Thiffault, John Herriges, Linda Cooley, Jennifer Gass, Ray Louie, Elliot Stolerman, Camerun Washington, Francesco Vetrini, Aiko Otsubo, Victoria M. Pratt, Erin Conboy, Kayla Treat, Nora Shannon, Jill A. Rosenfeld, Monte Westerfield, Michael Wangler, Shinya Yamamoto, Cigall Kadoch, Daryl A. Scott, Hugo J. Bellen , The American Journal of Human Genetics 2020 Nov 14:S0002-9297(20)30400-6. doi: 10.1016/j.ajhg.2020.11.003. *equal contribution.

The Why of YY1: Mechanisms of Transcriptional Regulation, Frontiers in Cell and Developmental Biology.
Thijs C. Verheul*, Levi van Hijfte*, Elena Perenthaler* and Tahsin Stefan Barakat# (2020)  8:592164. doi: 10.3389/fcell.2020.592164 *shared first authors, #corresponding author.

Malformations of cortical development, international consensus recommendations on diagnostic workup. 
Renske Oegema, Tahsin Stefan Barakat, Martina Wilke, Katrien Stouffs, Dina Amron, Eleanora Aronica, Nadia Bahi-Buisson, Valerio Conti, Andrew E. Fry, Tobias Geis, David Gomez Andres, Elena Parrini, Ivana Pogledic, Edith Said, Dorriete Soler, Luis M. Valor, Maha S. Zaki, Ghayda Mirzaa, William B Dobyns, Orly Reiner, Renzo Guerrini, Daniela Pilz, Ute Hehr, Richard J.Leventer, Anna Jansen, Grazia M.S. Mancini, Nataliya Di Donato  (2020)  Nature Reviews Neurology.  2020 Sep 7. doi: 10.1038/s41582-020-0395-6.

A second cohort of CHD3 patients expands the molecular mechanisms known to cause Snijders Blok-Campeau syndrome.
Theodore G Drivas, Joseph T Alaimo, Mariëlle Alders, Janine Altmüller, Tahsin Stefan Barakat, E Martina Bebin, Nicole L Bertsch, Patrick R Blackburn, Alyssa Blesson, Arjan M Bouman, Knut Brockmann, Perrine Brunelle, Margit Burmeister, Gregory M Cooper, Jonas Denecke, Anne Dieux-Coëslier, Holly Dubbs, Alejandro Ferrer, Danna Gal, Lauren E Grote, Lauren B Gunderson, Linda Hasadsri, Mahim Jain, Catherine Karamov, Beth Keena, Eric W Klee, Katja Kloth, Baiba Lace, Dong Li, Marina Macchiaiolo, Julien L Marcadier, Jeff M Milunsky, Divya Nair, Melanie P Napier, Xilma R Ortiz-Gonzalez, Pavel N. Pichurin, Jason Pinner, Zoe Powis, Chitra Prasad, Francesca Clementina Radio, Kristen J Rasmussen, Deborah L Renaud, Eric T Rush, Carol Saunders, Duygu Selcen, Ann R Seman, Deepali N Shinde, Erica D Smith, Thomas Smol, Lot Snijders Blok, Joan M Stoler, Sha Tang, Marco Tartaglia, Michelle L Thompson, Jiddeke M van de Kamp, Jingmin Wang, Dagmar Weise, Karin Weiss, Rixa Woitschach, Bernd Wollnik, Huifang Yan,Elaine H Zackai, Giuseppe Zampino, Philippe Campeau, Elizabeth Bhoj  (2020)  European Journal of Human Genetics. 2020 Jun 1. doi: 10.1038/s41431-020-0654-4.

Lessons learned from 40 novel PIGA patients and review of the literature.
Allan Bayat , Alexej Knaus, Manuela Pendziwiat, Alexandra Afenjar, Tahsin Stefan Barakat, Friedrich Bosch, Bert Callewaert, Patrick Calvas, Berten Ceulemans, Nicolas Chassing, Christel Depienne, Milda Endziniene, Carlos Feirrera, Caroline Fischinger, Moura de Souza, Cécile Freihuber, Shiva Ganesan, Svetlana Gataullina, Renzo Guerrini, Anne-Marie Guerrot, Lars Hansen, Aleksandra Jezela-Stanek, Caroline Karsenty, Anneke Kievit, Frank Kooy, Christian M. Korff, Johanne Kragh Hansen, Valérie Layet, Martin Larsen, Valery Layet, Gaetan Lesca, Kim McBride, Marije Meuwissen, Cyril Mignot, Martino Montomoli, Hannah Moore, Sophie Naudion, Caroline Nava, Marie-Christine Nougues, Elena Parrini, Matthew  Pastore, Jurgen H. Schelhaas, Steven Skinner, Krzysztoł Szczałuba, Ashley Thomas, Mads Thomassen, Lisbeth Tranebjærg, Marjon van Slegtenhorst, Lynne Wolfe, Dennis Lal, Elena Gardella, Lilian Bomme Ousager Tobias Brünger, Ingo Helbig, Peter Krawitz, Rikke S. Møller, (2020) Epilepsia.  2020 Jun;61(6):1142-1155. doi: 10.1111/epi.16545

Truncating mutations in YIF1B cause a progressive encephalopathy with various degrees of mixed movement disorder, microcephaly and epilepsy.
Mohammed AlMuhaizea, Rawan AlMass, Aljouhra AlHargan, Anoud AlBader, Eva Medico Salsench, Jude Howaidi, Jacie Ihinger, Peter Karachunski, Amber Begtrup, Monica Segura Castell, Peter Bauer, Aida Bertoli-Avella, Ibrahim H. Kaya, Jumanah AlSufayan, Laila AlQuait, Azizah Chedrawi, Stefan T. Arold, Dilek Colak, Tahsin Stefan Barakat# , Namik Kaya#. (2020). Acta Neuropathol. 2020 Jan 31. #corresponding authors.

Loss-of-function mutations in UDP-Glucose 6-Dehydrogenase cause recessive developmental epileptic encephalopathy.
Hengel H, Bosso-Lefèvre C, Grady G, Szenker-Ravi E, Li H, Pierce S, Lebigot E, Tan TT, Eio M, Narayanan G, Utami K, Yau M, Handal N, Deigendesch W, Keimer R, Marzouqa H, Gunay-Aygun M, Muriello M, Verhelst H, Weckhuysen S, Mahida S, Naidu S, Thomas T, Ying Lim J, Shien Tan E, Haye D, Willemsen M, Oegema R, Mitchell W, Pierson T, Andrews M, Willing M, Rodan L, Barakat TS, van Slegtenhorst M, Gavrilova R, Martinelli D, Gilboa T, Tamim A, Hashem M, AlSayed M, Abdulrahim M, Al-Owain M, Awaji A, Mahmoud A, Faqeih E, Al Asmar Ai, Algain S, Jad L,Aldhalaan H, Helbig I, Koolen D, Riess A, Kraegeloh-Mann I, Bauer P, Gulsuner S, Stamberger H, Ng A, Tang S, Tohari S, Keren B, Schultz-Rogers L, Klee E, Barresi S, Tartaglia M, Mor-Shaked H, Maddirevula S, Begtrup A, Telegraf Ai, Pfundt R, Schule R, Ciruna B, Bonnard C, Pouladi M, Stewart J, Claridge-Chang A, J Lefeber D, Alkuraya F, Mathuru A, Venkatesh B, Barycki J, Simpson M, Jamuar S, Schöls L and Reversade B. (2020). Nat Commun. 2020 Jan 30;11(1):595.

Loss of UGP2 in brain leads to a severe epileptic encephalopathy, emphasizing that bi-allelic isoform-specific start-loss mutations of essential genes can cause genetic diseases.
Perenthaler E, Nikoncuk A, Yousefi S, Berdowski WM, Alsagob M, Capo I, van der Linde HC, van den Berg P, Jacobs EH, Putar D, Ghazvini M, Aronica E, van IJcken WFJ, de Valk WG, Medici-van den Herik E, van Slegtenhorst M, Brick L, Kozenko M, Kohler JN, Bernstein JA, Monaghan KG, Begtrup A, Torene R, Al Futaisi A, Al Murshedi F, Mani R, Al Azri F, Kamsteeg EJ, Mojarrad M, Eslahi A, Khazaei Z, Darmiyan FM, Doosti M, Karimiani EG, Vandrovcova J, Zafar F, Rana N, Kandaswamy KK, Hertecant J, Bauer P, AlMuhaizea MA, Salih MA, Aldosary M, Almass R, Al-Quait L, Qubbaj W, Coskun S, Alahmadi KO, Hamad MHA, Alwadaee S, Awartani K, Dababo AM, Almohanna F, Colak D, Dehghani M, Mehrjardi MYV, Gunel M, Ercan-Sencicek AG, Passi GR, Cheema HA, Efthymiou S, Houlden H, Bertoli-Avella AM, Brooks AS, Retterer K, Maroofian R, Kaya N, van Ham TJ, Barakat TS#. (2019). Acta Neuropathol. 2019 Dec 9. #corresponding author.

De novo CLTC variants are associated with a variable phenotype from mild to severe intellectual disability, microcephaly, hypoplasia of the corpus callosum, and epilepsy.
Nabais Sá MJ, Venselaar H, Wiel L, Trimouille A, Lasseaux E, Naudion S, Lacombe D, Piton A, Vincent-Delorme C, Zweier C, Reis A, Trollmann R, Ruiz A, Gabau E, Vetro A, Guerrini R, Bakhtiari S, Kruer MC, Amor DJ, Cooper MS, Bijlsma EK, Barakat TS, van Dooren MF, van Slegtenhorst M, Pfundt R, Gilissen C, Willemsen MA, de Vries BBA, de Brouwer APM, Koolen DA. (2019). Genet Med. 2019 Nov 28.

Building Bridges Between the Clinic and the Laboratory: A Meeting Review – Brain Malformations: A Roadmap for Future Research.
Tamar Sapir, Tahsin Stefan Barakat, Mercedes F. Paredes, Tally Lerman-Sagie, Eleonora Aronica, Wlodzimierz Klonowski, Laurent Nguyen, Bruria Ben Zeev, Nadia Bahi-Buisson, Richard Leventer, Noa Rachmian and Orly Reiner. (2019). Front. Cell. Neurosci., 27 September 2019.

Beyond the Exome: The Non-coding Genome and Enhancers in Neurodevelopmental Disorders and Malformations of Cortical Development.
Elena Perenthaler, Soheil Yousefi, Eva Niggl and Tahsin Stefan Barakat. (2019). Front. Cell. Neurosci., 31 July 2019.

Exome Sequencing of a Primary Ovarian Insufficiency Cohort Reveals Common Molecular Etiologies for a Spectrum of Disease.
Jolly A, Bayram Y, Turan S, Aycan Z, Tos T, Abali ZY, Hacihamdioglu B, Coban Akdemir ZH, Hijazi H, Bas S, Atay Z, Guran T, Abali S, Bas F, Darendeliler F, Colombo R, Barakat TS, Rinne T1, White JJ, Yesil G, Gezdirici A, Gulec EY, Karaca E, Pehlivan D, Jhangiani SN, Muzny DM, Poyrazoglu S, Bereket A, Gibbs RA, Posey JE, Lupski JR. (2019). J Clin Endocrinol Metab. 2019 Aug 1;104(8):3049-3067.

The evolution of Great Apes has shaped the functional enhancers' landscape in human embryonic stem cells.
Glinsky G, Barakat TS. (2019). Stem Cell Res. 2019 May;37:101456.

REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice.
Gontan C, Mira-Bontenbal H, Magaraki A, Dupont C, Barakat TS, Rentmeester E, Demmers J, Gribnau J. (2018). Nat Commun. 2018 Nov 12;9(1):4752.

Functional Dissection of the Enhancer Repertoire in Human Embryonic Stem Cells.
Tahsin Stefan Barakat, Florian Halbritter, Man Zhang, André F. Rendeiro, Elena Perenthaler, Christoph Bock, and Ian Chambers. (2018). Cell Stem Cell. 2018 Aug 2; 23(2): 276–288.e8.

Dynamics of gene silencing during X inactivation using allele-specific RNA-seq.
Marks H, Kerstens HH, Barakat TS, Splinter E, Dirks RA, van Mierlo G, Joshi O, Wang SY, Babak T, Albers CA, Kalkan T, Smith A, Jouneau A, de Laat W, Gribnau J, Stunnenberg HG. (2015). Genome Biol. 2015 Aug 3;16:149.

Stable X chromosome reactivation in female human induced pluripotent stem cells.
Barakat TS, Ghazvini M, de Hoon B, Li T, Eussen B, Douben H, van der Linden R, van der Stap N, Boter M, Laven JS, Galjaard RJ, Grootegoed JA, de Klein A, Gribnau J. (2015). Stem Cell Reports. 2015 Feb 10;4(2):199-208.

Generation of knockout alleles by RFLP based BAC targeting of polymorphic embryonic stem cells.
Tahsin Stefan Barakat and Joost Gribnau. (2015). Methods Mol Biol. 2015;1227:143-80.

Combined DNA-RNA fluorescent in situ hybridization (FISH) to study X chromosome inactivation in differentiated female mouse embryonic stem cells.
Barakat TS, Gribnau J. (2014). J Vis Exp. 2014 Jun 14;(88).

The trans-activator RNF12 and cis-acting elements effectuate X chromosome inactivation independent of X-pairing.
Barakat TS, Loos F, van Staveren S, Myronova E, Ghazvini M, Grootegoed JA, Gribnau J. (2014). Mol Cell. 2014 Mar 20;53(6):965-78.

Structural and numerical changes of chromosome X in patients with esophageal atresia.
Brosens E, de Jong EM, Barakat TS, Eussen BH, D'haene B, De Baere E, Verdin H4, Poddighe PJ, Galjaard RJ, Gribnau J, Brooks AS, Tibboel D, de Klein A. (2014). Eur J Hum Genet. 2014 Sep;22(9):1077-84.

The pluripotency factor-bound intron 1 of Xist is dispensable for X chromosome inactivation and reactivation in vitro and in vivo.
Minkovsky A, Barakat TS, Sellami N, Chin MH, Gunhanlar N, Gribnau J, Plath K. (2013). Cell Rep. 2013 Mar 28;3(3):905-18.

X chromosome inactivation in the cycle of life.
Barakat TS, Gribnau J. (2012). Development. 2012 Jun;139(12):2085-9.

RNF12 initiates X-chromosome inactivation by targeting REX1 for degradation.
Gontan C, Achame EM, Demmers J, Barakat TS, Rentmeester E, van IJcken W, Grootegoed JA, Gribnau J. (2012). Nature. 2012 Apr 29;485(7398):386-90.

RNF12 controls embryonic stem cell fate and morphogenesis in zebrafish embryos by targeting Smad7 for degradation.
Zhang L, Huang H, Zhou F, Schimmel J, Pardo CG, Zhang T, Barakat TS, Sheppard KA, Mickanin C, Porter JA, Vertegaal AC, van Dam H, Gribnau J, Lu CX, ten Dijke P. Mol Cell. 2012 Jun 8;46(5):650-61.

Precise BAC targeting of genetically polymorphic mouse ES cells.
Barakat TS, Rentmeester E, Sleutels F, Grootegoed JA, Gribnau J. (2011). Nucleic Acids Res. 2011 Oct;39(18):e121.

RNF12 activates Xist and is essential for X chromosome inactivation.
Barakat TS, Gunhanlar N, Pardo CG, Achame EM, Ghazvini M, Boers R, Kenter A, Rentmeester E, Grootegoed JA, Gribnau. (2011). PLoS Genet. 2011 Jan 27;7(1):e1002001.

X chromosome inactivation and embryonic stem cells.
Barakat TS, Gribnau J. Adv Exp Med Biol. 2010;695:132-54.

A murine ESC-like state facilitates transgenesis and homologous recombination in human pluripotent stem cells.
Buecker C, Chen HH, Polo JM, Daheron L, Bu L, Barakat TS, Okwieka P, Porter A, Gribnau J, Hochedlinger K, Geijsen N. (2010). Cell Stem Cell. 2010 Jun 4;6(6):535-46.

X-changing information on X inactivation.
Barakat TS1, Jonkers I, Monkhorst K, Gribnau J. (2010). Exp Cell Res. 2010 Mar 10;316(5):679-87.

RNF12 is an X-Encoded dose-dependent activator of X chromosome inactivation.
Jonkers I, Barakat TS, Achame EM, Monkhorst K, Kenter A, Rentmeester E, Grosveld F, Grootegoed JA, Gribnau J. (2009). Cell. 2009 Nov 25;139(5):999-1011.

Books

Living with two X chromosomes: of mice and women : studies on the initiation mechanisms of X chromosome inactivation in stem cells and mouse models, and the role of RNF12 herein

Other publications

X-chromosome inactivation and its implications for human disease.
Joost Gribnau, Tahsin Stefan Barakat.

Can adrenaline safely be used in local anaesthesia involving distal extremities? A systematic review. Tahsin Stefan Barakat, Mariska de Pagter and Michel ten Have. (2015). Erasmus Journal of Medicine 4(2):15-19.

Collaborations

Collaboration within Erasmus MC

  • Grazia Mancini, Erasmus MC, Rotterdam
  • Tjakko van Ham, Erasmus MC, Rotterdam
  • Department of Cell Biology, Erasmus MC, Rotterdam
  • Academic Center for Stem Cells & Organoids in personalized and Regenerative medicine (SCORE).

Collaboration outside of Erasmus MC

Funding & Grants

The Barakat lab is supported by grants from:

  • The Dutch Research Council (ZonMW Veni)
  • The Brain & Behavior Research Foundation (NARSAD Young investigator Award)
  • Erasmus MC Fellowship 2017
  • Erasmus MC Human Disease Model Award 2018
  • Erasmus MC MRACE Pilot Grant 2018
  • EpilepsieNL

Previously, the principal investigator has been supported by:

  • MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual  Fellowships (IF-EF) from the European Union
  • EMBO Long-Term Fellowship
  • Niels Stensen Fellowship
  • Human Frontiers Science Project Long-Term Fellowship (Gratefully declined in favor of EMBO/MSCA)
  • American Society of Human Genetics (ASHG) /Charles J. Epstein Trainee Award 2015 for Excellence in Human Genetics Research – Winner
  • Beverly Kerr McKinnel Award 2012, of the International Society for Differentiation (ISD), for outstanding research as a PhD student

Career opportunities

We are always looking for talented people at all career steps. If you are interested in our research, and would like to discuss possibilities, please feel free to contact the PI T.Barakat directly by sending a CV.

Our team

Barakat team lab 2021

Alumni

Maria Eleftheriadou, 2020
Thomas Bossuyt, 2019
Darija Putar, 2018
Mariana Almeida, 2017-2018
Andrea Bellida Bude, 2017

Group of Tahsin Stefan Barakat

Barakat lab in 2017.

News

Amazing - Erasmus MC
Discoverers of very rare form of epilepsy now on track to treatment. 
Only 40 patients worldwide are known to have Barakat-Perenthaler syndrome, a severe untreatable form of epilepsy. Four years after discovering the disease, its Rotterdam namesakes are now on the trail of a treatment.

Amazing - Erasmus MC
Signposts for genetically unexplained brain disorders.
Genetics is more than just genes; this has been known for some time. But how do you find your way around the millions of pieces of DNA whose function is not yet known? Researchers at Erasmus MC took a big step in separating the wheat from the chaff during brain development.