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Research group/lab  |  P.I. Dr. S. (Stijn) Keereweer, P.I. Dr. D.E. (Denise) Hilling

Fluorescence-Guided Surgery Group

Fluorescence-guided surgery is an intraoperative optical imaging method that provides surgeons with real-time guidance for the identification and delineation of tumours or vital structures. This novel approach is being tested by different surgical disciplines within the Erasmus MC.

About our research group/lab

Our research

To focus our activities in this rapidly expanding research field, we have formed the Fluorescence-Guided Surgery Group. The group includes all researchers that are involved in this field, extending over 14 different disciplines. Our vision is that close collaboration will empower us as a group to facilitate more efficient and productive research lines. By forming trans-disciplinary partnerships, we are focusing on new clinical trials, improved coordination of the use of imaging facilities, coordinated and combined efforts for investments in new imaging devices and of research funding, and communication with secondary partners such as the pharmacy, medical ethical committee (METC), and the technology transfer office (TTO). This initiative flourishes through our close partnership with pioneering researchers at the Leiden University Medical Centre.

Our projects

  • Intraoperative tumor-specific fluorescence imaging of oral cancer (“Guided by Light” trial) and laryngeal/hypopharyngeal cancer (“STELLAR” trial)
  • Fluorescence imaging of recurrent and locally advanced rectal cancer using SGM-101
  • Complementary use of fluorescence-guided surgery and Raman spectroscopy
  • Vascular perfusion of anastomosis and reconstructive surgery

Key Publications

Real-time fluorescence imaging in intraoperative decision making for cancer surgery
Lorraine J Lauwerends, Pieter B A A van Driel, Robert J Baatenburg de Jong,
José A U Hardillo, Senada Koljenovic, Gerwin Puppels, Laura Mezzanotte,
Clemens W G M Löwik, Eben L Rosenthal, Alexander L Vahrmeijer, Stijn Keereweer.
The Lancet Oncology Published online March 22, 2021 doi.org/10.1016/S1470-2045(20)30600-8.

Fluorescence-guided surgery in colorectal cancer; A review on clinical results and future perspectives.
Galema HA, Meijer RPJ, Lauwerends LJ, Verhoef C, Burggraaf J, Vahrmeijer AL, Hutteman M, Keereweer S & Hilling DE.
Eur J Surg Oncol. 2021 Oct 9:S0748-7983(21)00755-1. doi: 10.1016/j.ejso.2021.10.005.

Current Intraoperative Imaging Techniques to Improve Surgical Resection of Laryngeal Cancer: A Systematic Review.
Lauwerends LJ, Galema HA, Hardillo JAU, Sewnaik A, Monserez D, van Driel PBAA, Verhoef C,
Baatenburg de Jong RJ, Hilling DE, Keereweer S.
Cancers (Basel). 2021 Apr 15;13(8):1895. doi: 10.3390/cancers13081895.


Funding & Grants

2020:      Maurits en Anna de Kock Stichting
PI: Stijn Keereweer, Tessa van Ginhoven
Title: “Improving surgical removal of oral cancer using fluorescence-guided surgery.”
Funding: €40.000,00


2019:      Daniel den Hoed Award / Erasmus MC Fund
PI: Stijn Keereweer (Personal Grant)
Post-doc: Hamed Abbasi
Title: “Improving surgical removal of oral cancer using a unique, innovative combination of fluorescence-guided surgery and Raman spectroscopy”
Funding: €250.000,00


2019:      Young Investigator Grant from the Dutch Cancer Society
PI: Stijn Keereweer (Personal Grant)
PhD student: L. Lauwerends
Title: “Guided by light: Optimizing surgical excision of oral cancer using real-time fluorescence imaging”
Funding: €497.935,00


2019:      Applied Efficiency Grant / Erasmus MC
PI: Dirk Grunhagen, Stijn Keereweer
PhD Student: E. Mulder
Title: “MOTOR pilot study - Ex Vivo Application of Multispectral Optoacoustic Tomography & Reflectance Spectroscopy in Lymph Nodes and Pigmented Tissue”
Funding: €86.108,00


2019:      Maurits en Anna de Kock Stichting
PI: Stijn Keereweer, Elisa Barroso
Title: “Improving surgical removal of oral cancer using an innovative combination of fluorescence-guided surgery and Raman spectroscopy.”
Funding: €36.370,00


2018:      Dutch Cancer Society Consortium Grant
PI: Denise Hilling, Kees Verhoef, Alexander Vahrmeijer (LUMC), Harm Rutten (CZE)
PhD student: Job van Kooten, Hidde Galema, Jan van Rees
Title: Tumour-specific near-infrared fluorescence imaging in locally advanced and recurrent rectal cancer
Funding: €1.071.553,00




Our team

  Department of Otorhinolaryngology:


Any questions or comments?

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